Journal article
A new non-degradative method to purify glycogen
Carbohydrate Polymers, Vol.147, pp.165-170
2016
PMID: 27178921
Abstract
Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any intrinsically-attached protein(s) is an essential prerequisite for studying this structural impairment. This requires an effective, non-degradative and efficient purification method to exclude the many other proteins present in liver. Proteins and glycogen have different ranges of molecular sizes. Despite the plethora of proteins that might still be present in significant abundance after other isolation techniques, SEC (size exclusion chromatography, also known as GPC), which separates by molecular size, should separate those extraneous to glycogen from glycogen with any intrinsically associated protein(s). A novel purification method is developed for this, based on preparative SEC following sucrose gradient centrifugation. Proteomics is used to show that the new method compares favourably with current methods in the literature. (C) 2016 Elsevier Ltd. All rights reserved.
Details
- Title
- A new non-degradative method to purify glycogen
- Authors
- Mitchell A Sullivan - University of QueenslandFei Gao - Huazhong University of Science and TechnologyBenjamin L Schulz - University of QueenslandRobert G. Gilbert (Corresponding Author) - University of Queensland
- Publication details
- Carbohydrate Polymers, Vol.147, pp.165-170
- Publisher
- Elsevier Ltd
- Date published
- 2016
- DOI
- 10.1016/j.carbpol.2016.04.009
- ISSN
- 1879-1344
- PMID
- 27178921
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035097402621
- Output Type
- Journal article
Metrics
6 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Chemistry, Applied
- Chemistry, Organic
- Polymer Science
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites