Journal article
A new high-prevalence LW antigen detected by an antibody in an Indigenous Australian homozygous for LWA c.309C>A variant
Vox Sanguinis, Vol.117(7), pp.958-965
2022
PMID: 35412682
Abstract
Background and objectives: The LW gene encodes the LW glycoprotein that carries the antigens of the LW blood group system. LW antigens are distinct from D antigen, however, they are phenotypically related and anti-LW antibodies are often mistaken as anti-D. An antibody was detected in an Australian patient of Aboriginal descent who consistently typed as LW(a+b-). This study aimed to describe the antibody recognizing a high-prevalence antigen on the LW glycoprotein.
Study design and methods: Samples from the patient and her four siblings were investigated. DNA was genotyped by single nucleotide polymorphism (SNP)-microarray and massively parallel sequencing (MPS) platforms. Red blood cells (RBCs) were phenotyped using standard haemagglutination techniques. Antibody investigations were performed using a panel of phenotyped RBCs from adults and cord blood cells.
Results: SNP-microarray and MPS genotyped all family members as LW*A/A, (c.299A), predicting LW(a+b-). In addition, a novel LW*A c.309C>A single nucleotide variant was detected in all family members. The patient and one of her siblings (M4) were LW c.309C>A homozygous. Antibody from the patient reacted positive to all reagent panel RBCs and cord blood cells but negative with RBCs from LW(a-b-), Rhnull and sibling M4. Antibody failed to react with RBCs treated with dithiothreitol.
Conclusion: Antibody detected in the patient recognized a novel high-prevalence antigen, LWEM, in the LW blood group system. LWEM-negative patients who developed anti-LWEM can be safely transfused with D+ RBCs, however, D- is preferred. Accurate antibody identification can help better manage allocation of blood products especially when D- RBCs are in short supply.
Details
- Title
- A new high-prevalence LW antigen detected by an antibody in an Indigenous Australian homozygous for LWA c.309C>A variant
- Authors
- Genghis H Lopez (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - LegacyBrett Wilson (Author) - Australian Red Cross Blood ServiceGlenda M Millard (Author) - Australian Red Cross Blood ServiceTanya L Cawthorne (Author) - Australian Red Cross Blood ServiceDianne E Grey (Author) - Pathwest Laboratory MedicineElizabeth A Fong (Author) - Pathwest Laboratory MedicineRobert L Flower (Author) - Australian Red Cross Blood ServiceCatherine A Hyland (Author) - Queensland University of TechnologyYew-Wah Liew (Author) - Australian Red Cross Blood Service
- Publication details
- Vox Sanguinis, Vol.117(7), pp.958-965
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2022
- DOI
- 10.1111/vox.13276
- ISSN
- 1423-0410
- PMID
- 35412682
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99629437302621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Hematology
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Source: InCites