A microplate enzyme immunoassay for detecting and measuring antibodies to Babesia bovis in cattle serum

D N Barry; B J Rodwell; Peter Timms; W McGregor

doi:10.1111/j.1751-0813.1982.tb02758.x

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A microplate enzyme immunoassay for detecting and measuring antibodies to Babesia bovis in cattle serum

Journal article

Peer reviewed

A microplate enzyme immunoassay for detecting and measuring antibodies to Babesia bovis in cattle serum

D N Barry, B J Rodwell, Peter Timms and W McGregor

Australian Veterinary Journal, Vol.59(5), pp.136-140

1982

DOI: https://doi.org/10.1111/j.1751-0813.1982.tb02758.x

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Abstract

A microplate enzyme immunoassay (EIA) is described for measuring IgG antibody to Babesia bovis in cattle serum. B. Bovis antibody status (whether positive or negative) and the amount of B. Bovis antibody (EIA score), were measured by comparison with reference serums. The EIA was shown to be specific for B. Bovis, and EIA score correlated well with EIA titre. Comparison of EIA with the Indirect Fluorescent Antibody Test (IFAT) showed more than 95% agreement between the methods and disagreement in only 1.6% of serum samples tested. The remaining 3.2% were positive by EIA and suspected positive by IFAT. The EIA was shown, by titrating positive serums, to be more sensitive than IFAT, which explained its tendency to detect more positive serums than IFAT. EIA detected B. bovis antibody in experimentally infected cattle by day 14 post infection (pi) and for at least 268 days pi. EIA score for B. bovis antibody in immune cattle increased significantly (p less than 0.05) following heterologous strain challenge.

Details

Title: A microplate enzyme immunoassay for detecting and measuring antibodies to Babesia bovis in cattle serum
Authors: D N Barry (Author) - Queensland Department of Primary Industries
B J Rodwell (Author) - Queensland Department of Primary Industries
Peter Timms (Author) - Queensland Department of Primary Industries
W McGregor (Author) - Queensland Department of Primary Industries
Publication details: Australian Veterinary Journal, Vol.59(5), pp.136-140
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 1982
DOI: 10.1111/j.1751-0813.1982.tb02758.x
ISSN: 0005-0423
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449621302621
Output Type: Journal article

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