A label-free potentiometric sensor principle for the detection of antibody-antigen interactions

M S Ozdemir; M Marczak; H Bohets; K Bonroy; D Roymans; L Stuyver; K Vanhoutte; M Pawlak; Eric Bakker

doi:10.1021/ac400514u

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A label-free potentiometric sensor principle for the detection of antibody-antigen interactions

Journal article

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A label-free potentiometric sensor principle for the detection of antibody-antigen interactions

M S Ozdemir, M Marczak, H Bohets, K Bonroy, D Roymans, L Stuyver, K Vanhoutte, M Pawlak and Eric Bakker

Analytical Chemistry, Vol.85(9), pp.4770-4776

2013

DOI: https://doi.org/10.1021/ac400514u

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Abstract

We report here on a new potentiometric biosensing principle for the detection of antibody-antigen interactions at the sensing membrane surface without the need to add a label or a reporter ion to the sample solution. This is accomplished by establishing a steady-state outward flux of a marker ion from the membrane into the contacting solution. The immunobinding event at the sensing surface retards the marker ion, which results in its accumulation at the membrane surface and hence in a potential response. The ion-selective membranes were surface-modified with an antibody against respiratory syncytial virus using click chemistry between biotin molecules functionalized with a triple bond and an azide group on the modified poly (vinyl chloride) group of the membrane. The bioassay sensor was then built up with streptavidin and subsequent biotinylated antibody. A quaternary ammonium ion served as the marker ion. The observed potential was found to be modulated by the presence of respiratory syncytial virus bound on the membrane surface. The sensing architecture was confirmed with quartz crystal microbalance studies, and stir effects confirmed the kinetic nature of the marker release from the membrane. The sensitivity of the model sensor was compared to that of a commercially available point-of-care test, with promising results.

Details

Title: A label-free potentiometric sensor principle for the detection of antibody-antigen interactions
Authors: M S Ozdemir (Author) - Janssen Research and Development, Belgium
M Marczak (Author) - Janssen Research and Development, Belgium
H Bohets (Author) - Octens BVBA, Belgium
K Bonroy (Author) - Janssen Infectious Diseases, Belgium
D Roymans (Author) - Janssen Infectious Diseases, Belgium
L Stuyver (Author) - Janssen Infectious Diseases, Belgium
K Vanhoutte (Author) - Janssen Research and Development, Belgium
M Pawlak (Author) - University of Geneva, Switzerland
Eric Bakker (Author) - University of Geneva, Switzerland
Publication details: Analytical Chemistry, Vol.85(9), pp.4770-4776
Publisher: American Chemical Society
Date published: 2013
DOI: 10.1021/ac400514u
ISSN: 0003-2700
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449021402621
Output Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Web Of Science research areas: Chemistry, Analytical