A high-throughput method for measuring critical thermal limits of leaves by chlorophyll imaging fluorescence

Pieter A. Arnold; Veronica F. Briceno; Kelli M. Gowland; Alexandra A. Catling; Leon A. Bravo; Adrienne B. Nicotra

doi:10.1071/FP20344

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A high-throughput method for measuring critical thermal limits of leaves by chlorophyll imaging fluorescence

Journal article

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A high-throughput method for measuring critical thermal limits of leaves by chlorophyll imaging fluorescence

Pieter A. Arnold, Veronica F. Briceno, Kelli M. Gowland, Alexandra A. Catling, Leon A. Bravo and Adrienne B. Nicotra

Functional Plant Biology , Vol.48(6), pp.634-646

2021

DOI: https://doi.org/10.1071/FP20344

PMID: 33663678

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Abstract

chlorophykll fluorescence

cold tolerance

ecophysiology

physiological ecology

temperature stress

Plant thermal tolerance is a crucial research area as the climate warms and extreme weather events become more frequent. Leaves exposed to temperature extremes have inhibited photosynthesis and will accumulate damage to PSII if tolerance thresholds are exceeded. Temperature-dependent changes in basal chlorophyll fluorescence (T-F-0) can be used to identify the critical temperature at which PSII is inhibited. We developed and tested a high-throughput method for measuring the critical temperatures for PSII at low (CTMIN) and high (CTMAX) temperatures using a Maxi-Imaging fluorimeter and a thermoelectric Peltier plate heating/cooling system. We examined how experimental conditions of wet vs dry surfaces for leaves and heating/cooling rate, affect CTMIN and CTMAX across four species. CTMAX estimates were not different whether measured on wet or dry surfaces, but leaves were apparently less cold tolerant when on wet surfaces. Heating/cooling rate had a strong effect on both CTMAX and CTMIN that was species-specific. We discuss potential mechanisms for these results and recommend settings for researchers to use when measuring T-F-0. The approach that we demonstrated here allows the high-throughput measurement of a valuable ecophysiological parameter that estimates the critical temperature thresholds of leaf photosynthetic performance in response to thermal extremes.

Details

Title: A high-throughput method for measuring critical thermal limits of leaves by chlorophyll imaging fluorescence
Authors: Pieter A. Arnold (Corresponding Author) - Australian National University
Veronica F. Briceno (Author) - Australian National University
Kelli M. Gowland (Author) - Australian National University
Alexandra A. Catling (Author) - Australian National University
Leon A. Bravo (Author) - Universidad de La Frontera
Adrienne B. Nicotra (Author) - Australian National University
Publication details: Functional Plant Biology , Vol.48(6), pp.634-646
Publisher: C S I R O Publishing
Date published: 2021
DOI: 10.1071/FP20344
ISSN: 1445-4416; 1445-4408
PMID: 33663678
Grant note: DP170101681 / Australian Research Council
Organisation Unit: Tropical Forests and People Research Centre
Language: English
Record Identifier: 99746598802621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Plant Sciences