A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a

N S Bende; S Dziemborowicz; M Mobli; Volker Herzig; J Gilchrist; J Wagner; G M Nicholson; G F King; F Bosmans

doi:10.1038/ncomms5350

Back

A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a

Journal article

Peer reviewed

A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a

N S Bende, S Dziemborowicz, M Mobli, Volker Herzig, J Gilchrist, J Wagner, G M Nicholson, G F King and F Bosmans

Nature Communications, Vol.5, 4350

2014

DOI: https://doi.org/10.1038/ncomms5350

Files and links (1)

url

https://doi.org/10.1038/ncomms5350View

Published Version

Abstract

β-Diguetoxin-Dc1a (Dc1a) is a toxin from the desert bush spider Diguetia canities that incapacitates insects at concentrations that are non-toxic to mammals. Dc1a promotes opening of German cockroach voltage-gated sodium (Nav) channels (BgNav1), whereas human Na v channels are insensitive. Here, by transplanting commonly targeted S3b-S4 paddle motifs within BgNav1 voltage sensors into Kv 2.1, we find that Dc1a interacts with the domain II voltage sensor. In contrast, Dc1a has little effect on sodium currents mediated by PaNav 1 channels from the American cockroach even though their domain II paddle motifs are identical. When exploring regions responsible for PaNa v 1 resistance to Dc1a, we identified two residues within the BgNav 1 domain II S1-S2 loop that when mutated to their PaNav 1 counterparts drastically reduce toxin susceptibility. Overall, our results reveal a distinct region within insect Nav channels that helps determine Dc1a sensitivity, a concept that will be valuable for the design of insect-selective insecticides.

Details

Title: A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a
Authors: N S Bende (Author)
S Dziemborowicz (Author)
M Mobli (Author)
Volker Herzig (Author) - University of Queensland
J Gilchrist (Author)
J Wagner (Author)
G M Nicholson (Author)
G F King (Author)
F Bosmans (Author)
Publication details: Nature Communications, Vol.5, 4350
Publisher: Nature Publishing Group
Date published: 2014
DOI: 10.1038/ncomms5350
ISSN: 2041-1723
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450826802621
Output Type: Journal article

Metrics

57 Record Views

72 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites