A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei

R Jayaraj; B Hales; L Vieberg; S Pizzutto; D C Holt; J M Rolland; R E O'Hehir; B J Currie; Shelley F Walton

doi:10.1016/j.diagmicrobio.2011.09.007

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A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei

Journal article

Peer reviewed

A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei

R Jayaraj, B Hales, L Vieberg, S Pizzutto, D C Holt, J M Rolland, R E O'Hehir, B J Currie and Shelley F Walton

Diagnostic Microbiology and Infectious Disease, Vol.71(4), pp.403-407

2011

DOI: https://doi.org/10.1016/j.diagmicrobio.2011.09.007

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Abstract

Sarcoptes scabiei

scabies

immunodiagnostic

IgE

allergen

Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50% accuracy. To develop more reliable diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA). Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar s 14.3 DELFIA showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s 14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice.

Details

Title: A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei
Authors: R Jayaraj (Author) - Charles Darwin University
B Hales (Author) - University of Western Australia
L Vieberg (Author) - Menzies School of Health Research, Darwin
S Pizzutto (Author) - Menzies School of Health Research, Darwin
D C Holt (Author) - Menzies School of Health Research, Darwin
J M Rolland (Author) - Monash University
R E O'Hehir (Author) - Monash University
B J Currie (Author) - Menzies School of Health Research, Darwin
Shelley F Walton (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Diagnostic Microbiology and Infectious Disease, Vol.71(4), pp.403-407
Publisher: Elsevier Inc.
Date published: 2011
DOI: 10.1016/j.diagmicrobio.2011.09.007
ISSN: 1879-0070
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
Language: English
Record Identifier: 99449883602621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Infectious Diseases; Microbiology

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