Journal article
A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys
Journal of Pharmacognosy and Phytotherapy, Vol.4(1), pp.6-11
2012
Abstract
New Zealand mānuka (Leptospermum scoparium) honey is known to exhibit non-peroxide antibacterial activity caused by the active ingredient methylglyoxal which arises by chemical conversion of dihydroxyacetone during honey maturation. This study determines whether methylglyoxal and dihydroxyacetone are present in Australian Leptospermum honeys. This research developed a rapid and sensitive high-performance liquid chromatographic method for the concurrent analysis of methylglyoxal and dihydroxyacetone in honeys. Both compounds were quantified as their O-(2, 3, 4, 5, 6-pentafluorobenzyl) hydroxylamine. HCl derivatives on single run reversed phase high-performance liquid chromatography with diode array detection. Four species of monofloral Leptospermum honeys sourced from Northern Rivers Region, New South Wales, Australia contained methylglyoxal and dihydroxyacetone. The highest methylglyoxal concentrations were found in Leptospermum polygalifolium honeys.
Details
- Title
- A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys
- Authors
- Sarah Windsor (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMatthew Pappalardo (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringPeter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringSimon Williams (Author) - University of Waikato, New ZealandM Manley-Harris (Author) - University of Waikato, New Zealand
- Publication details
- Journal of Pharmacognosy and Phytotherapy, Vol.4(1), pp.6-11
- Publisher
- Academic Journals
- Date published
- 2012
- DOI
- 10.5897/JPP11.025
- ISSN
- 2141-2502; 2141-2502
- Copyright note
- Copyright © 2012 Academic Journals. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 License.
- Organisation Unit
- School of Science and Engineering - Legacy; School of Education and Tertiary Access; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449623302621
- Output Type
- Journal article
- Research Statement
- false
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