A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys

Sarah Windsor; Matthew Pappalardo; Peter R Brooks; Simon Williams; M Manley-Harris

doi:10.5897/JPP11.025

Back

A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys

Journal article

Open access

Peer reviewed

A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys

Sarah Windsor, Matthew Pappalardo, Peter R Brooks, Simon Williams and M Manley-Harris

Journal of Pharmacognosy and Phytotherapy, Vol.4(1), pp.6-11

2012

DOI: https://doi.org/10.5897/JPP11.025

Files and links (2)

pdf

PDF - Published Version (Open Access)240.20 kBDownload View

Published VersionPDF - Published Version (Open Access)CC BY V3.0, Open Access

url

https://doi.org/10.5897/JPP11.025View

Published Version

Abstract

high-performance liquid chromatography

dihydroxyacetone

methylglyoxal

Leptospermum

manuka

New Zealand mānuka (Leptospermum scoparium) honey is known to exhibit non-peroxide antibacterial activity caused by the active ingredient methylglyoxal which arises by chemical conversion of dihydroxyacetone during honey maturation. This study determines whether methylglyoxal and dihydroxyacetone are present in Australian Leptospermum honeys. This research developed a rapid and sensitive high-performance liquid chromatographic method for the concurrent analysis of methylglyoxal and dihydroxyacetone in honeys. Both compounds were quantified as their O-(2, 3, 4, 5, 6-pentafluorobenzyl) hydroxylamine. HCl derivatives on single run reversed phase high-performance liquid chromatography with diode array detection. Four species of monofloral Leptospermum honeys sourced from Northern Rivers Region, New South Wales, Australia contained methylglyoxal and dihydroxyacetone. The highest methylglyoxal concentrations were found in Leptospermum polygalifolium honeys.

Details

Title: A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys
Authors: Sarah Windsor (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Matthew Pappalardo (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Peter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Simon Williams (Author) - University of Waikato, New Zealand
M Manley-Harris (Author) - University of Waikato, New Zealand
Publication details: Journal of Pharmacognosy and Phytotherapy, Vol.4(1), pp.6-11
Publisher: Academic Journals
Date published: 2012
DOI: 10.5897/JPP11.025
ISSN: 2141-2502
Organisation Unit: School of Science and Engineering - Legacy; School of Education and Tertiary Access; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449623302621
Output Type: Journal article

Metrics

490 File views/ downloads

3020 Record Views