A comparison of protocols for isolating and concentrating protein from the green seaweed Ulva ohnoi

A R Angell; Nicholas A Paul; R de Nys

doi:10.1007/s10811-016-0972-7

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A comparison of protocols for isolating and concentrating protein from the green seaweed Ulva ohnoi

Journal article

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A comparison of protocols for isolating and concentrating protein from the green seaweed Ulva ohnoi

A R Angell, Nicholas A Paul and R de Nys

Journal of Applied Phycology, Vol.29(2), pp.1011-1026

2017

DOI: https://doi.org/10.1007/s10811-016-0972-7

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Abstract

amino acid

macroalgae

protein

protein concentration

protein extraction

protein isolation

seaweed chlorophyta

ulva ohnoi

We compared protocols to isolate and concentrate protein from the green seaweed Ulva ohnoi. We quantified the effect of three factors on protein and essential amino acid yields and concentrations in protein isolates and residuals in a factorial experimental design. The three factors were starting material (as dry and milled or fresh and pulped), aqueous solvent-to-biomass ratio (20:1 or 5:1 v/w) and the incubation time in the aqueous solvent (incubated for 16 h at 30 °C or incubated for <1 min at ambient temperature). The protein isolation protocols increased the concentration of protein, total essential amino acids, methionine and lysine ~3 to 5-fold compared to whole U. ohnoi and were considerably more effective than the different protein concentrating combinations, which only increased protein and amino acid concentrations by 30-40 % in the residual biomass. The use of fresh and pulped biomass as the starting material, an incubation time of <1 min at ambient temperature and a low aqueous solution volume resulted in the highest protein isolate yield of 22 % of the protein found in seaweed. This study demonstrated that proteins from U. ohnoi were most effectively isolated by adopting protocols for terrestrial leaves compared to the protocols employed for seed crops as traditionally applied to seaweeds. © 2016 Springer Science+Business Media Dordrecht

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Title: A comparison of protocols for isolating and concentrating protein from the green seaweed Ulva ohnoi
Authors: A R Angell (Author) - James Cook University
Nicholas A Paul (Author) - James Cook University
R de Nys (Author) - James Cook University
Publication details: Journal of Applied Phycology, Vol.29(2), pp.1011-1026
Publisher: Springer Netherlands
Date published: 2017
DOI: 10.1007/s10811-016-0972-7
ISSN: 0921-8971
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99450456602621
Output Type: Journal article

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