A comparative study of the clonal diversity and virulence characteristics of uropathogenic Escherichia coli isolated from Australian and Turkish (Turkey) children and adults with urinary tract infections

Dylan Astley; Labolina Spang; Fatemeh Parnian; Tara Vollmerhausen; Mehmet Hora; Aycan Gundogdu; Mohammad Katouli

doi:10.18683/germs.2022.1324

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A comparative study of the clonal diversity and virulence characteristics of uropathogenic Escherichia coli isolated from Australian and Turkish (Turkey) children and adults with urinary tract infections

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A comparative study of the clonal diversity and virulence characteristics of uropathogenic Escherichia coli isolated from Australian and Turkish (Turkey) children and adults with urinary tract infections

Dylan Astley, Labolina Spang, Fatemeh Parnian, Tara Vollmerhausen, Mehmet Hora, Aycan Gundogdu and Mohammad Katouli

Germs, Vol.12(2), pp.214-230

2022

DOI: https://doi.org/10.18683/germs.2022.1324

PMCID: PMC9719378

PMID: 36504619

Appears in UniSC Diversity and Inclusion Research Collection

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Abstract

uropathogenic Escherichia coli

urinary tract infection

Turkey

Australia

UniSC Diversity Area - Life Stages

Introduction: The virulence-associated gene (VAG) repertoire and clonal organization of uropathogenic Escherichia coli (UPEC) strains is influenced by host demographic, geographic locale, and the setting of urinary tract infection (UTI). Nevertheless, a direct comparison of these features among Australian and Turkish UPEC remains unexplored. Accordingly, this study investigated the clonal composition and virulence characteristics of a collection of UPEC isolated from Australian and Turkish UTI patients. Methods: A total of 715 UPEC strains isolated from Australian (n=361) and Turkish (n=354) children and adults with hospital (HA)- and community-acquired (CA)-UTIs were included in this study. Typing of the strains using RAPD-PCR and PhPlate fingerprinting grouped all strains into 25 clonal groups (CGs). CG representatives were phylogrouped and screened for the presence of 18 VAGs associated with extraintestinal pathogenic E. coli. Results: Turkish UPEC strains were characterized by high clonal diversity and predominance of the phylogroup D, while few distinct clonal groups with phylogenetic group B2 backgrounds dominated among the Australian strains. Twelve identical CGs were shared between ≥1 patient group from either country. Australian strains, particularly those isolated from children with HA-UTI, showed higher virulence potential than their Turkish counterparts, carrying significantly more genes associated with adhesion, iron acquisition and capsule biosynthesis. Conclusions: This study identified identical CGs of UPEC causing HA- and CA-UTIs among Australian and Turkish UTI patients. These CGs frequently carried VAGs associated with adhesion, iron acquisition, immune evasion, and toxin production, which may contribute to their ability to disseminate internationally and to cause UTI.

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Title: A comparative study of the clonal diversity and virulence characteristics of uropathogenic Escherichia coli isolated from Australian and Turkish (Turkey) children and adults with urinary tract infections
Authors: Dylan Astley (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Labolina Spang (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Fatemeh Parnian (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Tara Vollmerhausen (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Mehmet Hora (Author) - Erciyes University
Aycan Gundogdu (Author) - Erciyes University
Mohammad Katouli (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Publication details: Germs, Vol.12(2), pp.214-230
Publisher: Institutul de Bolinfectioase "Matei Bals"
Date published: 2022
DOI: 10.18683/germs.2022.1324
ISSN: 2248-2997
PMID: 36504619; PMC9719378
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99659497702621
Output Type: Journal article

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