Journal article
A combination of PhP typing and β-D-glucuronidase gene sequence variation analysis for differentiation of Escherichia coli from humans and animals
Canadian Journal of Microbiology, Vol.61(6), pp.409-416
2015
Abstract
We investigated the usefulness of the β-D-glucuronidase gene variance in E. coli as a MST tool using a novel algorithm for comparison of sequences from a pre-screened set of host specific isolates using a high resolution PhP typing method. A total of 65 common biochemical phenotypes belonging to 318 E. coli strains isolated from humans, domestic and wild animals were analysed for nucleotide variations at 10 loci along a 518bp fragment of the 1812bp β-D-glucuronidase gene. Neighbour-Joining analysis of loci variations revealed 86 (76.8%) human isolates and 91.2% of animal isolates were correctly identified. Pair-wise hierarchical clustering improved assignment; where 92 (82.1%) human and 204 (99%) animal strains were assigned to their respective cluster. Our data shows initial typing of isolates and selection of common types from different hosts prior to analysis of the β-D-glucuronidase gene sequence improves source identification. We also concluded that numerical profiling of the nucleotide variations can be used as a valuable approach to differentiate human from animal E. coli. This study signifies the usefulness of the β-D-glucuronidase gene as a marker for differentiating human faecal pollution from animal sources.
Details
- Title
- A combination of PhP typing and β-D-glucuronidase gene sequence variation analysis for differentiation of Escherichia coli from humans and animals
- Authors
- Nicole Masters (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMelodie Christie (Author) - Griffith UniversityMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringHelen Stratton (Author) - Griffith University
- Publication details
- Canadian Journal of Microbiology, Vol.61(6), pp.409-416
- Publisher
- N R C Research Press
- Date published
- 2015
- DOI
- 10.1139/cjm-2015-0048
- ISSN
- 0008-4166
- Organisation Unit
- School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449268502621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Immunology
- Microbiology
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Source: InCites