A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus

Thomas M. Banks; Tianfang Wang; Quinn P. Fitzgibbon; Gregory G. Smith; Tomer Ventura

doi:10.3390/ijms231911752

Back

A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus

Journal article

Open access

Peer reviewed

A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus

Thomas M. Banks, Tianfang Wang, Quinn P. Fitzgibbon, Gregory G. Smith and Tomer Ventura

International Journal of Molecular Sciences, Vol.23(19), pp.1-16

2022

DOI: https://doi.org/10.3390/ijms231911752

PMCID: PMC9569428

PMID: 36233053

Files and links (2)

pdf

A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus2.74 MBDownload View

Published Version Open Access CC BY V4.0

url

https://doi.org/10.3390/ijms231911752View

Published Version

Abstract

RNA interference

achelata

decapod crustaceans

gene silencing mechanism

transcriptomic analysis

RNA interference (RNAi) has been widely utilised in many invertebrate models since its discovery, and in a majority of instances presents as a highly efficient and potent gene silencing mechanism. This is emphasized in crustaceans with almost all taxa having the capacity to trigger effective silencing, with a notable exception in the spiny lobsters where repeated attempts at dsRNA induced RNAi have demonstrated extremely ineffective gene knockdown. A comparison of the core RNAi machinery in transcriptomic data from spiny lobsters (Panulirus ornatus) and the closely related slipper lobsters (Thenus australiensis, where silencing is highly effective) revealed that both lobsters possess all proteins involved in the small interfering and microRNA pathways, and that there was little difference at both the sequence and domain architecture level. Comparing the expression of these genes however demonstrated that T. australiensis had significantly higher expression in the transcripts encoding proteins which directly interact with dsRNA when compared to P. ornatus, validated via qPCR. These results suggest that low expression of the core RNAi genes may be hindering the silencing response in P. ornatus, and suggest that it may be critical to enhance the expression of these genes to induce efficient silencing in spiny lobsters.

Details

Title: A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus
Authors: Thomas M. Banks (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Tianfang Wang (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Quinn P. Fitzgibbon (Author) - University of Tasmania
Gregory G. Smith (Author) - University of Tasmania
Tomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Publication details: International Journal of Molecular Sciences, Vol.23(19), pp.1-16
Publisher: MDPI AG
Date published: 2022
DOI: 10.3390/ijms231911752
ISSN: 1422-0067
PMID: 36233053; PMC9569428
Copyright note: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Grants: The ARC Research Hub for Sustainable Lobster Aquaculture, IH190100014, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99681396802621
Output Type: Journal article

Metrics

329 File views/ downloads

66 Record Views

6 Times Cited - Web of Science

See more details

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: SDGs from InCites