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A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus2.74 MB
Published VersionCC BY V4.0, Open Access
Journal article
A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus
International Journal of Molecular Sciences, Vol.23(19), pp.1-16
2022
PMID: 36233053
Abstract
RNA interference (RNAi) has been widely utilised in many invertebrate models since its discovery, and in a majority of instances presents as a highly efficient and potent gene silencing mechanism. This is emphasized in crustaceans with almost all taxa having the capacity to trigger effective silencing, with a notable exception in the spiny lobsters where repeated attempts at dsRNA induced RNAi have demonstrated extremely ineffective gene knockdown. A comparison of the core RNAi machinery in transcriptomic data from spiny lobsters (Panulirus ornatus) and the closely related slipper lobsters (Thenus australiensis, where silencing is highly effective) revealed that both lobsters possess all proteins involved in the small interfering and microRNA pathways, and that there was little difference at both the sequence and domain architecture level. Comparing the expression of these genes however demonstrated that T. australiensis had significantly higher expression in the transcripts encoding proteins which directly interact with dsRNA when compared to P. ornatus, validated via qPCR. These results suggest that low expression of the core RNAi genes may be hindering the silencing response in P. ornatus, and suggest that it may be critical to enhance the expression of these genes to induce efficient silencing in spiny lobsters.
Details
- Title
- A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock Lobster Panulirus ornatus
- Authors
- Thomas M. Banks (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTianfang Wang (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationQuinn P. Fitzgibbon (Author) - University of TasmaniaGregory G. Smith (Author) - University of TasmaniaTomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- International Journal of Molecular Sciences, Vol.23(19), pp.1-16
- Publisher
- MDPI AG
- Date published
- 2022
- DOI
- 10.3390/ijms231911752
- ISSN
- 1422-0067; 1661-6596
- PMID
- 36233053
- Copyright note
- © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99681396802621
- Output Type
- Journal article
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