Journal article
A Proteomic Analysis for the Red Seaweed Asparagopsis taxiformis
Biology, Vol.12(2), pp.1-14
2023
PMCID: PMC9952816
PMID: 36829446
Abstract
The red seaweed Asparagopsis taxiformis is a promising ruminant feed additive with anti-methanogenic properties that could contribute to global climate change solutions. Genomics has provided a strong foundation for in-depth molecular investigations, including proteomics. Here, we investigated the proteome of A. taxiformis (Lineage 6) in both sporophyte and gametophyte stages, using soluble and insoluble extraction methods. We identified 741 unique non-redundant proteins using a genome-derived database and 2007 using a transcriptome-derived database, which included numerous proteins predicted to be of fungal origin. We further investigated the genome-derived proteins to focus on seaweed-specific proteins. Ontology analysis indicated a relatively large proportion of ion-binding proteins (i.e., iron, zinc, manganese, potassium and copper), which may play a role in seaweed heavy metal tolerance. In addition, we identified 58 stress-related proteins (e.g., heat shock and vanadium-dependent haloperoxidases) and 44 photosynthesis-related proteins (e.g., phycobilisomes, photosystem I, photosystem II and ATPase), which were in general more abundantly identified from female gametophytes. Forty proteins were predicted to be secreted, including ten rhodophyte collagen-alpha-like proteins (RCAPs), which displayed overall high gene expression levels. These findings provide a comprehensive overview of expressed proteins in A. taxiformis, highlighting the potential for targeted protein extraction and functional characterisation for future biodiscovery.
Details
- Title
- A Proteomic Analysis for the Red Seaweed Asparagopsis taxiformis
- Authors
- Zubaida P. Patwary (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationMin Zhao (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTianfang Wang (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationNicholas A. Paul (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringScott F. Cummins (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Biology, Vol.12(2), pp.1-14
- Publisher
- MDPI AG
- Date published
- 2023
- DOI
- 10.3390/biology12020167
- ISSN
- 2079-7737
- PMID
- 36829446; PMC9952816
- Copyright note
- © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Organisation Unit
- Cancer Research Cluster; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99702098902621
- Output Type
- Journal article
Metrics
247 File views/ downloads
85 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- International collaboration
- Web Of Science research areas
- Biology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: SDGs from InCites