A Proteomic Analysis for the Red Seaweed Asparagopsis taxiformis

Zubaida P. Patwary; Min Zhao; Tianfang Wang; Nicholas A. Paul; Scott F. Cummins

doi:10.3390/biology12020167

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A Proteomic Analysis for the Red Seaweed Asparagopsis taxiformis

Journal article

Open access

Peer reviewed

A Proteomic Analysis for the Red Seaweed Asparagopsis taxiformis

Zubaida P. Patwary, Min Zhao, Tianfang Wang, Nicholas A. Paul and Scott F. Cummins

Biology, Vol.12(2), pp.1-14

2023

DOI: https://doi.org/10.3390/biology12020167

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Abstract

Asparagopsis taxiformis

seaweed

proteomics

mass spectrometry

sporophyte

gametophyte

The red seaweed Asparagopsis taxiformis is a promising ruminant feed additive with anti-methanogenic properties that could contribute to global climate change solutions. Genomics has provided a strong foundation for in-depth molecular investigations, including proteomics. Here, we investigated the proteome of A. taxiformis (Lineage 6) in both sporophyte and gametophyte stages, using soluble and insoluble extraction methods. We identified 741 unique non-redundant proteins using a genome-derived database and 2007 using a transcriptome-derived database, which included numerous proteins predicted to be of fungal origin. We further investigated the genome-derived proteins to focus on seaweed-specific proteins. Ontology analysis indicated a relatively large proportion of ion-binding proteins (i.e., iron, zinc, manganese, potassium and copper), which may play a role in seaweed heavy metal tolerance. In addition, we identified 58 stress-related proteins (e.g., heat shock and vanadium-dependent haloperoxidases) and 44 photosynthesis-related proteins (e.g., phycobilisomes, photosystem I, photosystem II and ATPase), which were in general more abundantly identified from female gametophytes. Forty proteins were predicted to be secreted, including ten rhodophyte collagen-alpha-like proteins (RCAPs), which displayed overall high gene expression levels. These findings provide a comprehensive overview of expressed proteins in A. taxiformis, highlighting the potential for targeted protein extraction and functional characterisation for future biodiscovery.

Details

Title: A Proteomic Analysis for the Red Seaweed Asparagopsis taxiformis
Authors: Zubaida P. Patwary (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Min Zhao (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Tianfang Wang (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Nicholas A. Paul (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Scott F. Cummins (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Publication details: Biology, Vol.12(2), pp.1-14
Publisher: MDPI AG
Date published: 2023
DOI: 10.3390/biology12020167
ISSN: 2079-7737
Copyright note: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Grants: Harnessing seaweed genes to mitigate methane emissions from livestock, DP200103013, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: Cancer Research Cluster; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99702098902621
Output Type: Journal article