Journal article
A Novel Ferritin-Core Analog Is a Safe and Effective Alternative to Oral Ferrous Iron for Treating Iron Deficiency during Pregnancy in Mice
The Journal of Nutrition, Vol.152(3), pp.714-722
2022
PMID: 34625812
Abstract
Background Many women enter pregnancy with iron stores that are insufficient to maintain maternal iron balance and support fetal development and consequently, often require iron supplements. However, the side effects associated with many currently available iron supplements can limit compliance. Objective This study aimed to test the safety and efficacy of a novel nanoparticulate iron supplement, a dietary ferritin analog termed iron hydroxide adipate tartrate (IHAT), in pregnant mice. Methods Female C57BL/6 mice were maintained on either an iron-deficient or a control diet for 2 wk prior to timed mating to develop iron-deficient or iron-sufficient pregnancy models, respectively. Mice from each model were then gavaged daily with 10 mg iron/kg body weight as either IHAT or ferrous sulfate, or with water only, beginning on embryonic day (E) 4.5. Mice were killed on E18.5 and maternal iron and hematological parameters were measured. The expression of genes encoding iron transporters and oxidative stress markers in the duodenum and placenta were determined, along with hepatic expression of the gene encoding the iron regulatory hormone hepcidin and fetal iron. Results Oral IHAT and ferrous sulfate were equally effective at increasing maternal hemoglobin (20.2% and 16.9%, respectively) and hepatic iron (30.2% and 29.3%, respectively), as well as total fetal iron (99.7% and 83.8%, respectively), in iron-deficient pregnant mice compared with those gavaged with water only, with no change in oxidative stress markers seen with either treatment. However, there was a significant increase in the placental expression of the oxidative stress marker heme oxygenase 1 in iron-replete pregnant mice treated with ferrous sulfate when compared with iron-replete pregnant mice gavaged with IHAT (96.9%, P <0.05). Conclusions IHAT has proved a safe and effective alternative to oral ferrous sulfate in mice, and it has potential for treating iron deficiency in human pregnancy.
Details
- Title
- A Novel Ferritin-Core Analog Is a Safe and Effective Alternative to Oral Ferrous Iron for Treating Iron Deficiency during Pregnancy in Mice
- Authors
- Sheridan L. Helman (Author) - QIMR Berghofer Medical Research InstituteSarah J. Wilkins (Author) - QIMR Berghofer Medical Research InstituteDaniel R. McKeating (Author) - Griffith UniversityAnthony Perkins (Author) - Griffith UniversityJames S. M. Cuffe (Author) - University of QueenslandGunter Hartel (Author) - QIMR Berghofer Medical Research InstituteNuno Faria (Author) - University of CambridgeJonathan J. Powell (Author) - University of CambridgeGregory J. Anderson (Author) - QIMR Berghofer Medical Research InstituteDavid M. Frazer (Author) - QIMR Berghofer Medical Research Institute
- Publication details
- The Journal of Nutrition, Vol.152(3), pp.714-722
- Publisher
- Oxford University Press
- DOI
- 10.1093/jn/nxab363
- ISSN
- 1541-6100
- PMID
- 34625812
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy; School of Health
- Language
- English
- Record Identifier
- 99685294202621
- Output Type
- Journal article
Metrics
57 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Nutrition & Dietetics
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites