Journal article
A Multi-subunit chlamydial vaccine induces antibody and cell-mediated immunity in immunized koalas (phascolarctos cinereus): Comparison of three different adjuvants
American Journal of Reproductive Immunology, Vol.63(2), pp.161-172
2010
Abstract
Problem: Chlamydial infections represent a major threat to the survival of the koala. Infections caused by Chlamydia pecorum cause blindness, infertility, pneumonia and urinary tract infections and represent a threat to the survival of the species. Little is known about the immune response in koalas, or the safety of commonly used adjuvants for induction of protective systemic and mucosal immunity. Method of study: In the present study, we immunized 18 healthy female koalas subcutaneously with a combination of three chlamydial antigens [major outer membrane protein (MOMP), NrdB and TC0512 (Omp85)] mixed with one of three different adjuvants [Alhydrogel, Immunostimulating Complex (ISC) and TiterMax Gold]. Results: All adjuvants induced strong neutralizing IgG responses in plasma against the three antigens with prolonged responses lasting more than 270 days seen in Alhydrogel and ISC immunized animals. Cloacal IgG responses lasting >270 days were also induced in ISC-immunized animals. Chlamydia-specific peripheral blood mononuclear cell proliferative responses were elicited by both Alhydrogel and ISC, and these lasted >270 days in the ISC group. Conclusion: The data show that a multi-subunit chlamydial vaccine, given subcutaneously, can elicit Chlamydia-specific cell-mediated and antibody responses in the koala demonstrating that the development of a protective vaccine is feasible.
Details
- Title
- A Multi-subunit chlamydial vaccine induces antibody and cell-mediated immunity in immunized koalas (phascolarctos cinereus): Comparison of three different adjuvants
- Authors
- A J Carey (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of TechnologyG Rawlinson (Author) - Lone Pine Koala Sanctuary, AustraliaJ Brumm (Author) - Queensland University of TechnologyK Nilsson (Author) - Lone Pine Koala Sanctuary, AustraliaJ M Harris (Author) - Queensland University of TechnologyK W Beagley (Author) - Queensland University of Technology
- Publication details
- American Journal of Reproductive Immunology, Vol.63(2), pp.161-172
- Publisher
- Wiley-Blackwell Publishing Inc.
- Date published
- 2010
- DOI
- 10.1111/j.1600-0897.2009.00776.x
- ISSN
- 1046-7408
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448908402621
- Output Type
- Journal article
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- Immunology
- Reproductive Biology
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