A Crown-of-Thorns Seastar recombinant relaxin-like gonad-stimulating peptide triggers oocyte maturation and ovulation

Meaghan Smith; Chieu Hoang Dinh; Joseph Aizen; Benjamin Mos; Cherie Motti; Abigail Elizur; Scott F Cummins

doi:10.1016/j.ygcen.2019.05.009

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A Crown-of-Thorns Seastar recombinant relaxin-like gonad-stimulating peptide triggers oocyte maturation and ovulation

Journal article

Open access

Peer reviewed

A Crown-of-Thorns Seastar recombinant relaxin-like gonad-stimulating peptide triggers oocyte maturation and ovulation

Meaghan Smith, Chieu Hoang Dinh, Joseph Aizen, Benjamin Mos, Cherie Motti, Abigail Elizur and Scott F Cummins

General and Comparative Endocrinology, Vol.281, pp.41-48

2019

DOI: https://doi.org/10.1016/j.ygcen.2019.05.009

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Abstract

Crown-of-Thorns Seastar

oocyte maturation

recombinant RGP

echinoderm

The Acanthaster planci species-complex [Crown-of-Thorns Seastar (COTS)] are highly fecund echinoderms that exhibit population outbreaks on coral reef ecosystems worldwide, including the Australian Great Barrier Reef. A better understanding of the COTS molecular biology is critical towards efforts in controlling outbreaks and assisting reef recovery. In seastars, the heterodimeric relaxin-like gonad stimulating peptide (RGP) is responsible for triggering a neuroendocrine cascade that regulates resumption of oocyte meiosis prior to spawning. Our comparative RNA-seq analysis indicates a general increase in RGP gene expression in the female radial nerve cord during the reproductive season. Also, the sensory tentacles demonstrate a significantly higher expression level than radial nerve cord. A recombinant COTS RGP, generated in a yeast expression system, is highly effective in inducing oocyte germinal vesicle breakdown (GVBD), followed by ovulation from ovarian fragments. The findings of this study provide a foundation for more in-depth molecular analysis of the reproductive neuroendocrine physiology of the COTS and the RGP.

Details

Title: A Crown-of-Thorns Seastar recombinant relaxin-like gonad-stimulating peptide triggers oocyte maturation and ovulation
Authors: Meaghan Smith (Author) - University of the Sunshine Coast
Chieu Hoang Dinh (Author) - University of the Sunshine Coast
Joseph Aizen (Author) - University of the Sunshine Coast
Benjamin Mos (Author) - Southern Cross University
Cherie Motti (Author) - Australian Institute of Marine Science
Abigail Elizur (Author) - University of the Sunshine Coast
Scott F Cummins (Author) - University of the Sunshine Coast
Publication details: General and Comparative Endocrinology, Vol.281, pp.41-48
Publisher: Academic Press
Date published: 2019
DOI: 10.1016/j.ygcen.2019.05.009
ISSN: 0016-6480
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450656002621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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