A Comparative Study of Assay Performance of Commercial Hepatitis E Virus Enzyme-Linked Immunosorbent Assay Kits in Australian Blood Donor Samples

Ashish C Shrestha; Robert L P Flower; Clive R Seed; Susan L Stramer; Helen M Faddy

doi:10.1155/2016/9647675

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A Comparative Study of Assay Performance of Commercial Hepatitis E Virus Enzyme-Linked Immunosorbent Assay Kits in Australian Blood Donor Samples

Journal article

Open access

Peer reviewed

A Comparative Study of Assay Performance of Commercial Hepatitis E Virus Enzyme-Linked Immunosorbent Assay Kits in Australian Blood Donor Samples

Ashish C Shrestha, Robert L P Flower, Clive R Seed, Susan L Stramer and Helen M Faddy

Journal of Blood Transfusion, Vol.2016, 9647675

2016

DOI: https://doi.org/10.1155/2016/9647675

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Abstract

Clinical Sciences

Cardiovascular Medicine and Haematology

Immunology

Hepatitis E virus (HEV) is transfusion-transmissible and therefore poses a risk to blood transfusion safety. Seroprevalence studies are useful for estimating disease burden and determining risk factors. Considerable variability in the sensitivity of HEV antibody detection assays exists. This study aimed to compare the performances of commercially available HEV enzyme-linked immunosorbent assays (ELISA) in Australian blood donor samples. Plasma samples that tested positive () or negative () for HEV IgG (Wantai HEV IgG ELISA) were selected. Of the 194 HEV IgG positive samples, 4 were positive for HEV IgM (Wantai HEV IgM ELISA). All samples were tested with the MP Diagnostics: HEV IgG ELISA, total (IgG, IgM, and IgA) HEV antibody ELISA, and HEV IgM ELISA. Of the 194 Wantai HEV IgG positive samples, 92 (47%) tested positive with the MP Diagnostics HEV IgG ELISA () and 126 (65%) with MP Diagnostics total HEV antibody assay (). There was poor agreement between Wantai and MP Diagnostics HEV IgM assays. This study demonstrated poor agreement between the assays tested. These observations are consistent with previous reports demonstrating significant variability between HEV ELISAs, highlighting that results of HEV serology should be interpreted with caution.

Details

Title: A Comparative Study of Assay Performance of Commercial Hepatitis E Virus Enzyme-Linked Immunosorbent Assay Kits in Australian Blood Donor Samples
Authors: Ashish C Shrestha (Author) - Australian Red Cross Blood Service
Robert L P Flower (Author) - Australian Red Cross Blood Service
Clive R Seed (Author) - Australian Red Cross Blood Service
Susan L Stramer (Author) - American Red Cross, United States
Helen M Faddy (Author) - Australian Red Cross Blood Service
Publication details: Journal of Blood Transfusion, Vol.2016, 9647675; 6
Publisher: Hindawi Publishing Corporation
Date published: 2016
DOI: 10.1155/2016/9647675
ISSN: 2090-9187
Copyright note: Copyright © 2016 Ashish C. Shrestha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450839202621
Output Type: Journal article

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