5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability

Georgia Rose Kafer; Xuan Li; Takuro Horii; Isao Suetake; Shoji Tajima; Izuho Hatada; Peter Mark Carlton

doi:10.1016/j.celrep.2016.01.035

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5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability

Journal article

Open access

Peer reviewed

5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability

Georgia Rose Kafer, Xuan Li, Takuro Horii, Isao Suetake, Shoji Tajima, Izuho Hatada and Peter Mark Carlton

Cell Reports, Vol.14(6), pp.1283-1292

2016

DOI: https://doi.org/10.1016/j.celrep.2016.01.035

PMID: 26854228

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Abstract

5-hydroxymethylcytosine (5hmC) is a DNA base created during active DNA demethylation by the recently discovered TET enzymes. 5hmC has essential roles in gene expression and differentiation. Here, we demonstrate that 5hmC also localizes to sites of DNA damage and repair. 5hmC accumulates at damage foci induced by aphidicolin and microirradiation and colocalizes with major DNA damage response proteins 53BP1 and γH2AX, revealing 5hmC as an epigenetic marker of DNA damage. Deficiency for the TET enzymes eliminates damage-induced 5hmC accumulation and elicits chromosome segregation defects in response to replication stress. Our results indicate that the TET enzymes and 5hmC play essential roles in ensuring genome integrity.

Details

Title: 5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability
Authors: Georgia Rose Kafer (Author) - Kyoto University
Xuan Li (Author) - Kyoto University
Takuro Horii (Author) - Gunma University
Isao Suetake (Author) - The University of Osaka
Shoji Tajima (Author) - The University of Osaka
Izuho Hatada (Author) - Gunma University
Peter Mark Carlton (Author) - Kyoto University
Publication details: Cell Reports, Vol.14(6), pp.1283-1292
Publisher: Cell Press
Date published: 2016
DOI: 10.1016/j.celrep.2016.01.035
ISSN: 2211-1247
PMID: 26854228
Organisation Unit: School of Health - Biomedicine; Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99512808802621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Cell Biology

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