Dissertation
The Biocontrol Potential of Termite-gut-associated Actinomycetes against Phytopathogenic Fungus Pyrrhoderma noxium
University of the Sunshine Coast, Queensland
Doctor of Philosophy, University of the Sunshine Coast, Queensland
2024
DOI:
https://doi.org/10.25907/00819
Abstract
Fungal phytopathogens significantly impact global agricultural systems, threatening crop productivity and food security. Pyrrhoderma noxium is a phytopathogenic fungus that is causing the invasive disease of brown root-rot in many of the amenity trees within Brisbane city, Australia. In order to develop effective mitigation strategies, biological control offers safer and more environmentally-friendly alternatives to conventional chemical control interventions. This research aimed to bioprospect termite gut-associated actinomycetes metabolomes as a source of antifungal secondary metabolites to biologically control Pyrrhoderma noxium.
In the first stage of this study, the termite gut-associated actinomycetes were screened for antifungal activity with 15 isolates found to be active against the Pyrrhoderma noxium (P. noxium). The actinomycete isolates underwent molecular characterisation and a majority were identified as streptomycetes including the active isolates identified in the antifungal screening. Further screening was conducted through fermentation studies of the active isolates to elicit and extract their secondary metabolites. Untargeted MS-based metabolomic methods provided insight into the abundant chemical classes within these fermentation extracts and gave tentative annotations. Polyketides were amongst the most dominant with active compounds within classes of coumarins, flavonoids, macrolides and cyclic peptides identified.
Details
- Title
- The Biocontrol Potential of Termite-gut-associated Actinomycetes against Phytopathogenic Fungus Pyrrhoderma noxium
- Authors
- Cherrihan Adra - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Contributors
- D. İpek Kurtböke (Supervisor) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringTrong Tran (Supervisor) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Awarding institution
- University of the Sunshine Coast, Queensland
- Degree awarded
- Doctor of Philosophy
- Publisher
- University of the Sunshine Coast, Queensland
- DOI
- 10.25907/00819
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99999997102621
- Output Type
- Dissertation
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