Dissertation
Investigation of the molecular basis of chemosensory communication in aquatic environments using a host (Biomphalaria) and parasite (Schistosoma) system
University of the Sunshine Coast, Queensland
Doctor of Philosophy, University of the Sunshine Coast
2017
DOI:
https://doi.org/10.25907/00631
Abstract
In aquatic environments, the vast majority of invertebrate animals live in darkness, and therefore the chemosensory sense is of critical importance. To date, limited information is known about the molecular regulatory systems involved in aquatic chemosensation. Host-parasite interactions provide an ideal experimental model system for investigation into the molecular basis of chemosensory communication in aquatic environments, where the parasite seeks out a host whilst the host attempts to evade the parasite; these two behaviors are driven by chemosensory mechanisms. In this thesis research, we chose a focus on the interplay between the intermediate snail host, Biomphalaria glabrata, and the parasitic trematode, Schistosoma mansoni, providing us with the supreme host-parasite interplay for studies to elucidate aquatic olfactory signaling. Besides, new findings in this area may help to ameliorate the associated human disease, schistosomiasis, which kills up to 200,000 people annually. We first analyzed the B. glabrata genome database to explore putative chemosensory receptors, and report that there are up to 242 intact chemosensory G-protein coupled receptor (GPCR) genes that belong to 14 subfamilies and ionotropic glutamate receptor (IR) supergene families. An animal's ability to smell is dependent on a set proteins (encoded in the genes) that must be produced and housed within anatomical structures (chemosensory organs) that allow for external detection and neural processing to the higher brain centers. In situ hybridization was used to reveal that at least some candidate chemosensory receptors are expressed within sensory epithelia of the tentacle, strongly suggestive of a role in odorant perception.
Details
- Title
- Investigation of the molecular basis of chemosensory communication in aquatic environments using a host (Biomphalaria) and parasite (Schistosoma) system
- Authors
- Di Liang
- Contributors
- Scott F Cummins (Supervisor)
- Awarding institution
- University of the Sunshine Coast
- Degree awarded
- Doctor of Philosophy
- Publisher
- University of the Sunshine Coast, Queensland
- DOI
- 10.25907/00631
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450440602621
- Output Type
- Dissertation
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