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DataCC0 V1.0, Open Access
DataCC0 V1.0, Open
Dataset
Structural models of Cheiracanthium punctorium spider toxins with putative defensive function (CSTX-type and phospholipase A2)
Dryad
Dryad
2025
Abstract
Spider venom is an important but evolutionarily poorly understudied functional trait. In this study, we have sequenced multiple venom gland transcriptomes from various spiders and analyzed their venom profile. The transcriptomes were generated via Illumina TruSeq RNA Sample Prep Kit v2 or TruSeq Stranded mRNA Library Prep Kit (paired-end, 151-bp read length), sequenced with Macrogen (Korea) using different Illumina chemistries an assembled de novo using a pipeline incorporating Trinity v2.13.2/2.15.1 as well as rnaSPAdes v3.15.5. Identified toxin precursors were annotated using InterProScan v5.61-93.0/5.69-101.0 and a DIAMOND v2.0.15/2.1.9 blastp search against the public available databases VenomZone, UniProtKB/Swiss-Prot Tox-Prot, UniProtKB/Swiss-Prot and UniProtKB/TrEMBL v2022_05/2024_04 was performed. Our analysis revealed the presence of novel double-domain toxins from the CSTX family and some phospholipase A2 toxins sequence-wise resembling homolgs from honeybees and scorpions, indicating functional similarity. In order to explore the architecture of the CSTX toxins and whether the observed phospholipase A2 sequence similarities have structural repercussions, we utilized structural predictions. Therefore, Alphafold 3 in the Galaxy platform was used to generate 3D models of the proteins. Sequences were submitted online and structures were predicted using default settings. The resulting model was downloaded and visualized using ChimeraX. The results indeed suggested a high structural similarity of phospholipases from spider, honeybee and scorpion venom which renders them as likely functionally related, supposedly acting in defense and illustrated a complex structure of novel CSTX toxins. The dataset herein presented contains the models of the chosen spider toxins stemming from the Nurses thorn finger (Cheiracanthium punctorium), as well as the most similar ones from honeybees and scorpions.
Details
- Title
- Structural models of Cheiracanthium punctorium spider toxins with putative defensive function (CSTX-type and phospholipase A2)
- Authors
- Tim Lüddecke (Data Collector) - Fraunhofer Institute for Molecular Biology and Applied EcologyJosephine Dresler (Data Collector) - Fraunhofer Institute for Molecular Biology and Applied EcologySabine Hurka (Data Collector) - Fraunhofer Institute for Molecular Biology and Applied EcologyVolker Herzig (Data Collector) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Dryad
- Format
- 7 x .cif; 1 README file; Total 517 KB
- Publisher
- Dryad
- Date published
- 2025
- DOI
- 10.5061/dryad.fn2z34v7t
- Grant note
- LOEWE-TBG / Hessian Ministry for Science and the Arts (https://ror.org/00zd5gr55)
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991173178402621
- Output Type
- Dataset
Metrics
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