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Data from: Development and proof of concept evaluation for a low-resource compatible Chikungunya virus diagnostic
Vol.8 September 2025
Dryad
2025
Abstract
Chikungunya virus (CHIKV) is a positive sense RNA Alphavirus that continues to pose major public health threats throughout the world. CHIKV is primarily transmitted via the Aedes genus mosquito; however, it has also exhibited transmission routes via blood transfusion and vertical transmission (mother to child). With only one approved vaccine thus far and no approved medicines, early detection is crucial in mitigating CHIKV outbreaks. Here, we designed and evaluated a sensitive and specific CHIKV diagnostic using reverse transcription-recombinase aided amplification (RT-RAA) coupled lateral flow strip detection (LFD) targeting a highly conserved region of the CHIKV E1 gene. Our results demonstrate that using our simple sample preparation reagent (TNA-Cifer-E) can inactivate live CHIKV in two minutes at room temperature, whilst also sustaining viable viral RNA. Our specificity analysis demonstrates that the Iso-CHIKV-Dx does not detect any closely related Alphaviruses nor any of the common co-circulating Flaviviruses. Proof-of-concept evaluation using urine spiked with CHIKV exhibited that in CHIKV-infected urine samples, this Iso-CHIKV-Dx can detect as low as 570 copies/μL of CHIKV RNA in 30 minutes under isothermal conditions. Contrary to conventional RT-qPCR, our Iso-CHIKV-Dx does not require expensive machinery, advanced instrumentation or extensively trained personnel. Further performance comparisons also show that this Iso-CHIKV-Dx is four times faster than conventional RNA isolation and RT-qPCR. As such, pre-clinical evaluation demonstrates that this Iso-CHIKV-Dx has the potential to act as a robust, point of care CHIKV diagnostic that could prove to be highly beneficial in place of, or in the absence of RT-qPCR.
Details
- Title
- Data from: Development and proof of concept evaluation for a low-resource compatible Chikungunya virus diagnostic
- Authors
- Rickyle Balea (Data Collector) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringAlberto A. Amarilla (Data Collector) - The University of QueenslandJody Hobson-Peters (Data Collector) - The University of QueenslandJoanne Macdonald (Data Collector) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringAndreas Suhbier (Data Collector) - QIMR Berghofer Medical Research InstituteVasilli M. Kasimov (Data Collector) - Griffith UniversityDaniel Watterson (Data Collector) - The University of QueenslandNina M. Pollak (Data Collector) - University of the Sunshine Coast, Queensland, Centre for BioinnovationDavid J. McMillan (Data Collector) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Vol.8 September 2025
- Format
- 10 files; Total: 1.09 MB
- Publisher
- Dryad
- Date published
- 2025
- DOI
- 10.5061/dryad.547d7wmmf
- Grants
- A single rapid field test for detecting Wolbachia, malaria and dengue in caught material, OPP1140133, Gates Foundation (United States, Seattle) - BMGF
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991162245202621
- Output Type
- Dataset
Metrics
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