Conference presentation
Effects of Ontogenic aging on rooting of Eucalyptus pellita cuttings and the use of Near-Infrared Spectral Analysis (NIRA) as a tool for predicting rooting potential
USC Research Conference, 2013 (Sunshine Coast, Australia, 01-Jul-2013–05-Jul-2013)
University of the Sunshine Coast
2013
Abstract
Effects of ontogenic ageing such as decreased stand productivity and reduced rooting competence in the production of cuttings can have significant implications for clonal forestry. This presentation reports on the results of two separate experiments undertaken to investigate the effects of ontogenic aging on rooting potential and the potential of NIRA as a predictive tool in clonal propagation. Rooting potential was measured in cuttings set from E.pellita clones of different ontogenic ages. Cuttings from nine year old hedges captured from selection-age trees had an average rooting amongst clones of 25.8% compared with 88.3% for cuttings from genetically related, seedlings-derived clonal hedges. Statistical analysis showed that differences between clones and 'age' treatments were statistically significant (p = 01) as was the interaction between these treatments. Root protrusion from the container cell base was not evident in cuttings from seedling-derived hedges until two weeks after setting, and up to four weeks from cuttings taken from 'aged' hedges, demonstrating a temporal aspect of ontogenic aging effect on rooting. However, peak root emergence was five weeks after setting irrespective of the age of the hedge material. Assays of endogenous plant hormones Indole-3-acetic acid (IAA) and Abscisic acid (ABA)) showed that there were significant differences between age treatments in levels of ABA (p = 01) but not IAA (p = 0.061). A second plant hormone assay was used in a regression analysis with near-infrared spectra acquired from leaves of cuttings set in a subsequent experiment. Results indicate a promising potential of NIRA to predict endogenous IAA (calibration r2 = .072, validation r2 = .66) but less power in predicting ABA concentration (calibration r2 = .055, validation r2 = .46) using first-derivative transformed data.
Details
- Title
- Effects of Ontogenic aging on rooting of Eucalyptus pellita cuttings and the use of Near-Infrared Spectral Analysis (NIRA) as a tool for predicting rooting potential
- Authors
- Paul Warburton (Author) - University of the Sunshine Coast, Queensland, Forest Industries Research Centre
- Conference details
- USC Research Conference, 2013 (Sunshine Coast, Australia, 01-Jul-2013–05-Jul-2013)
- Publisher
- University of the Sunshine Coast
- Date published
- 2013
- Copyright note
- Copyright © 2013 the author. Reproduced with the permission of the copyright holder.
- Organisation Unit
- Forest Industries Research Centre
- Language
- English
- Record Identifier
- 99448612602621
- Output Type
- Conference presentation
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