Diversity and Community Structure of Aerobic Methanotrophs in Sediments Associated with Hydrocarbon Seeps in the Tropical Timor Sea, Australia

Kenneth Wasmund; G Abell; D G Bourne; L Bodrossy; K A Burns; D Ipek Kurtboke

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Diversity and Community Structure of Aerobic Methanotrophs in Sediments Associated with Hydrocarbon Seeps in the Tropical Timor Sea, Australia

Kenneth Wasmund, G Abell, D G Bourne, L Bodrossy, K A Burns and D Ipek Kurtboke

International Symposium on Microbial Ecology (ISME), 12th (Cairns, Australia, 17-Aug-2008–22-Aug-2008)

International Society for Microbial Ecology

2008

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Abstract

Microbiology

methanotrophs

Timor Sea

real-time PCR

The diversity and community structure of aerobic methanotrophs in sediments associated with methane emitting hydrocarbon seeps in the tropical Timor Sea was studied by analysis of particulate methane monooxygenase (pmoA) gene diversity and distribution using real-time PCR, clone libraries and pmoA diagnostic microarray analysis. Real-time PCR showed overall highest numbers of methanotrophs in surface sediments associated with an intense active seep area. Clone libraries of pmoA genes recovered from surface (0-1 cm) and shallow sub-surface (4-5 cm) sediments revealed diverse pmoA phylotypes dominated by type Ib affiliated methanotrophs distantly related to cultivated representatives of the Methylocaldum and Methylococcus genera. Many sequences were significantly divergent from previously described phylotypes (≤91% amino acid and ≤86% nucleotide sequence identities), suggesting the occurrence of extensive novel methanotrophic taxa. All phylotypes were most closely related to phylotypes previously recovered from marine environments. Phylogenetic analysis suggested that the majority of phylotypes recovered in this study form a divergent cluster of marine specialized methanotrophs. Community structures of methanotrophs in surface versus sub-surface sediments were different. Clone libraries revealed some clusters of sequences were dominated by sequences recovered from either environment, while microarray analysis showed clear trends in intensity of probe signals in surface and sub-surface sediments.

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Title: Diversity and Community Structure of Aerobic Methanotrophs in Sediments Associated with Hydrocarbon Seeps in the Tropical Timor Sea, Australia
Authors: Kenneth Wasmund (Author) - Australian Institute of Marine Science
G Abell (Author) - Austrian Research Centers GmbH, Department of Bioresources, Seibersdorf, Austria
D G Bourne (Author) - Australian Institute of Marine Science
L Bodrossy (Author) - Austrian Research Centers GmbH, Department of Bioresources, Seibersdorf, Austria
K A Burns (Author) - Australian Institute of Marine Science
D Ipek Kurtboke (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Conference details: International Symposium on Microbial Ecology (ISME), 12th (Cairns, Australia, 17-Aug-2008–22-Aug-2008)
Publisher: International Society for Microbial Ecology
Date published: 2008
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering
Language: English
Record Identifier: 99449696102621
Output Type: Conference poster

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