Conference poster
Development of DArT markers for ultra-high-throughput genotyping of eucalypts
IUFRO Tree Biotechnology Conference, 2007 (Ponta Delgada, Portugal, 03-Jun-2007–08-Jun-2007)
IUFRO
2007
Abstract
Diversity Arrays Technology (DArT) could provide a low cost, high-throughput genotyping tool for eucalypts (at 1000s of polymorphic loci for approximately 30 euros per sample). The technology has been shown to work well in eucalypts. The development of DArT for eucalypts will help overcome two of the main problems in molecular tree breeding: the high cost of genotyping and the low genome coverage offered by most marker systems. This project aims to develop generic DArT arrays across several eucalypt species in the anticipation that enough markers will be polymorphic to perform genome-wide fingerprinting, population genetics, linkage mapping or phylogenetics, in any eucalypt species. Two arrays totalling 13,824 DArT markers will be developed across eight species; E. grandis, E. urophylla, E. globulus, E. nitens, E. camaldulensis, E. cladocalyx, E. pilularis and Corymbia variagata. These will be tested for their repeatability and cross-species utility through screening of six mapping families, each from different species and also by completing a phylogenetic analysis of eucalypts using samples from over 80 species sequenced for ITS. In order to increase the genome coverage of commercially important species and make possible high-resolution linkage mapping and comparative mapping, a second phase of DArT development will be started in August 2007. This will aim to develop an additional array of 6,912 DArT markers for the first four species listed above using germplasm from breeding programs contributed by project partners. We seek partners to help fund this second phase. Partners would need to contribute a minimum of $2,500 AUD and provide six DNA samples by August 2007. By including their genetic material in the process of marker discovery, partners will maximize the potential information content of the final DArT array for their own studies. Those interested in participating should contact René Vaillancourt by July 2007.
Details
- Title
- Development of DArT markers for ultra-high-throughput genotyping of eucalypts
- Authors
- R E Vaillancourt (Author) - University of TasmaniaDorothy A Steane (Author) - University of TasmaniaA Kilian (Author) - Diversity Arrays Technology Pty Ltd, AustraliaA A Myburg (Author) - University of Pretoria, South Africa
- Conference details
- IUFRO Tree Biotechnology Conference, 2007 (Ponta Delgada, Portugal, 03-Jun-2007–08-Jun-2007)
- Publisher
- IUFRO
- Date published
- 2007
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99450132102621
- Output Type
- Conference poster
Metrics
6 File views/ downloads
350 Record Views