Basement membrane thickening around capillaries in skeletal muscles as hallmark of human peripheral arterial disease

O Baum; C Malik; B Hoier; Meegan A Walker; Christopher D Askew; K Bansbo; Y Hellsten; H Hoppeler

Conference poster

Basement membrane thickening around capillaries in skeletal muscles as hallmark of human peripheral arterial disease

O Baum, C Malik, B Hoier, Meegan A Walker, Christopher D Askew, K Bansbo, Y Hellsten and H Hoppeler

Advances in the cellular and molecular biology of angiogenesis conference, 2011 (Birmingham, United Kingdom, 27-Jun-2011–29-Jun-2011)

2011

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Abstract

Cardiovascular Medicine and Haematology

To determine whether the thickening of the basement membrane (BM) is demonstrated in early stages of peripheral arterial disease (PAD; Fontaine grades IIa and IIb; patients suffering from intermittent claudication (IC)), we collected vastus lateralis muscle (VL) biopsies from eight IC patients and nine control subjects. Two resin blocks were obtained from each VL biopsy and subjected to morphometric analysis. 1-μm thick Toluidine Blue-stained sections were examined by light microscopy at 63x magnification. Both the capillary-to-muscle fiber-ratio (-23%) and the capillary density (-19%) were significantly lower (p ≤ 0.05) in the VL biopsies of the IC patients than in those of the controls. In contrast, the mean cross-sectional muscle fiber area in the VL biopsies differed only 3% (p less than 0.05) between the two cohorts. The ultrastructure of 18-20 capillaries (including their peri-vascular surrounding) per block was displayed by transmission electron microscopy at magnification of 17.000x. The absolute mean VL capillary area occupied by endothelial cells (+20%) and the basement membrane (+50%) but not of pericytes (+10%) and the lumen (-5%) was significantly altered (p ≤ 0.05) in the IC patients if compared to the control subjects. Furthermore, the mean volume occupied by the capillaries in toto was 19% larger (p ≤ 0.05) in the VL of the IC patients than the control subjects. Our analysis reveals intermittent claudication to be accompanied by an enlargement of the peri-capillary basement membrane in skeletal muscles.

Details

Title: Basement membrane thickening around capillaries in skeletal muscles as hallmark of human peripheral arterial disease
Authors: O Baum (Author) - University of Bern, Switzerland
C Malik (Author) - University of Bern, Switzerland
B Hoier (Author) - University of Copenhagen, Denmark
Meegan A Walker (Author) - Queensland University of Technology
Christopher D Askew (Author) - Queensland University of Technology
K Bansbo (Author) - University of Copenhagen, Denmark
Y Hellsten (Author) - University of Copenhagen, Denmark
H Hoppeler (Author) - University of Bern, Switzerland
Conference details: Advances in the cellular and molecular biology of angiogenesis conference, 2011 (Birmingham, United Kingdom, 27-Jun-2011–29-Jun-2011)
Date published: 2011
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99449836602621
Output Type: Conference poster

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