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Autophosphonylating peptides as the scavengers of nerve gas agents
Conference poster

Autophosphonylating peptides as the scavengers of nerve gas agents

Z Zhu, S-X Deng, Joanne Macdonald, M Vinogradov and D W Landry
American Chemical Society (ACS) National Meeting, 235th (New Orleans, United States, 06-Apr-2008–10-Apr-2008)
2008
url
http://oasys2.confex.com/acs/235nm/techprogram/P1150730.HTMView
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Abstract

Biochemistry and Cell Biology
Organophosphorus-based inhibitors of the serine esterase acetylcholinesterase, long used as agents for chemical warfare, are extremely toxic. Butyrylcholinesterase (BuChE) is the leading clinical scavenger of nerve agents but low enzymatic turnover rates and a high equivalent weight as a stoichiometric scavenger render maintenance of sufficient BuChE blood levels difficult. We now report development of autocatalytic, low molecular weight peptide-based stoichiometric scavengers. Serine-containing single-peptide-per-bead combinatorial libraries were screened by FACS sarin labeled with Oregon Green as the fluorescent probe. Twenty-four hits were identified and sequenced. Synthesis of soluble peptide, observed reaction with a non-volatile sarin analog and independent synthesis of the products confirmed the hit.

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