Conference paper
Comparison of DAS-ELISA and RT-PCR for the detection of dasheen mosaic virus (DsMV) in taro (Colocasia esculenta (L.) Schott)
National Agriculture Symposium 2020 Proceedings: "Agriculture Resilience to Calamities - Now and for Future", pp.49-56
National Agriculture Symposium: Agriculture Resilience to Calamities - Now and for Future, 2020 (Pacific Harbour, Fiji, 03-Dec-2020 - 04-Dec-2020)
Fiji Institute of Agricultural Science
2020
Abstract
Commercially available DAS-ELISA and RT-PCR were compared to determine the best method for the detection of Dasheen Mosaic Virus (DsMV), genus Potyvirus, from taro (C. esculenta). Fifty taro plants were randomly selected from the Koronivia Research Station (KRS) field germplasm collection and were tested for DsMV with DAS-ELISA and RT-PCR. 36% of the field samples were tested DsMV-positive using RT-PCR. In contrast, only 20% were determined as DsMV-positive using DAS-ELISA. Sensitivity in DsMV detection was 10 times higher(up to 10-3dilutions) using RT_PCR compared to that of DAS-ELISA (up to 10-2diluƟons). Despite the high sensitivity of RT-PCR, routine analysis is not feasible as the reagents are expensive, require more technical expertise and require purpose-built infrastructures compared to DASELISA. Therefore, DAS-ELISA is still an important tool that can be used for routine screening of DsMV at national clean seed systems while, RT-PCR can be used at genebank level, which require the highest level of sensitivity and accuracy, to ensure safe taro germplasm exchange.
Details
- Title
- Comparison of DAS-ELISA and RT-PCR for the detection of dasheen mosaic virus (DsMV) in taro (Colocasia esculenta (L.) Schott)
- Authors
- Amit Sukal (Corresponding Author) - Centre for the Pacific Crops and Trees (CePaCT) - Suva, FijiMary Taylor (Author) - University of the Sunshine Coast, Queensland, Faculty of Science, Health, Education and EngineeringSemi Cakaunitavuki (Author) - Centre for the Pacific Crops and Trees (CePaCT) - Suva, FijiGuofo Luo (Author) - Centre for the Pacific Crops and Trees (CePaCT) - Suva, FijiAnjeela Johkan (Author) - Ministry of Education, Heritage & Arts (Fiji) - MEHA
- Contributors
- Shalendra Prasad (Editor) - Ministry of AgricultureSalesh Kumar (Editor) - Fiji National UniversityAradhana Deesh (Editor) - Ministry of Agriculture
- Publication details
- National Agriculture Symposium 2020 Proceedings: "Agriculture Resilience to Calamities - Now and for Future", pp.49-56
- Conference details
- National Agriculture Symposium: Agriculture Resilience to Calamities - Now and for Future, 2020 (Pacific Harbour, Fiji, 03-Dec-2020 - 04-Dec-2020)
- Publisher
- Fiji Institute of Agricultural Science
- Organisation Unit
- Faculty of Science, Health, Education and Engineering; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99666998002621
- Output Type
- Conference paper
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