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Actin adsorbed on HOPG and Mica substrates: Characterization and manipulation by atomic force microscopy
Conference paper   Peer reviewed

Actin adsorbed on HOPG and Mica substrates: Characterization and manipulation by atomic force microscopy

D V Nicolau, Gregory S Watson, C Cahill, Jolanta A Blach and S Myhra
Technical Proceedings of the 2004 Nanotechnology Conference and Trade Show, Vol.1, pp.103-105
Nanotechnology Conference and Trade Show (Nanotech), 2004 (Boston, United States, 07-Mar-2004–11-Mar-2004)
Computational Publications
2004
url
http://www.nsti.org/publications/Nanotech/2004/pdf/B1-27.pdfView
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Abstract

actin atomic force microscope
Actin deposited onto a hydrophobic graphite surface forms paracrystalline rafts consisting of F-actin filaments. Those filaments retain their internal coherence during manipulation by an atomic force microscope tip. However, the filaments can be 'herded' systematically in a direction perpendicular to their long axis, due to weak interaction with the substrate. Actin deposited onto a hydrophilic mica surface, on the other hand, tend to become immobilized due to strong out-of-plane interactions. Circular ordered actin features can be formed at preferred sites defined by the intersection of interfaces at micro-bubbles with the substrate. The observations may have applications for devices based on dynamics of molecular motors.

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