AFM analysis of the formation of DNA aggregates on polymeric biochips

P D Sawant; Gregory S Watson; S Myhra; D Nicolau

doi:10.1117/12.584142

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AFM analysis of the formation of DNA aggregates on polymeric biochips

Conference paper

Peer reviewed

AFM analysis of the formation of DNA aggregates on polymeric biochips

P D Sawant, Gregory S Watson, S Myhra and D Nicolau

Proceedings of SPIE - International Society for Optical Engineering, Vol.5651, pp.153-159

Biomedical Applications of Micro- and Nanoengineering II, 2004 (Sydney, Australia, 13-Dec-2004–15-Dec-2004)

SPIE: International Society for Optical Engineering

2005

DOI: https://doi.org/10.1117/12.584142

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Abstract

atomic force microscopy

DNA hybridization

DNA immobilization

DNA microarrays

fractal dimensions

aggregates

binding energy

copolymers

DNA

microprocessor chips

olefins

surface properties

The demand for polymer-based DNA microarrays will increase because of their cost-effectiveness, biocompatibility and easy processing. However not all polymers are ideal substrates because of different chemical interactions of polymeric substrates with the DNA molecules. Results from AFM analysis of DNA immobilised on polymeric surfaces are evaluated using fractality, Gaussian distribution and lateral force imaging. It has been found that the nanosize defects in the substrate, such as poly-1-lysine, plays an important role in the growth of DNA aggregates in a vertical direction, whereas the covalent binding of DNA molecules on NHS-functionalised cyclo-olefin copolymer leads to the lateral growth of DNA aggregates.

Details

Title: AFM analysis of the formation of DNA aggregates on polymeric biochips
Authors: P D Sawant (Author) - Swinburne University of Technology
Gregory S Watson (Author) - Griffith University
S Myhra (Author) - Griffith University
D Nicolau (Author) - Swinburne University of Technology
Contributors: Dan V Nicolau (Editor)
Publication details: Proceedings of SPIE - International Society for Optical Engineering, Vol.5651, pp.153-159
Conference details: Biomedical Applications of Micro- and Nanoengineering II, 2004 (Sydney, Australia, 13-Dec-2004–15-Dec-2004)
Publisher: SPIE: International Society for Optical Engineering
Date published: 2005
DOI: 10.1117/12.584142
ISBN: 9780819456113
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99449238702621
Output Type: Conference paper

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