The chemical reactivity and structure of collagen studied by neutron diffraction

Tim J Wess; L Wess; A Miller

doi:10.1007/978-1-4615-5847-7_32

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$The chemical reactivity and structure of collagen studied by neutron diffraction$

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The chemical reactivity and structure of collagen studied by neutron diffraction

Tim J Wess, L Wess and A Miller

Neutrons in Biology, pp.369-383

Basic Life Sciences (BLSC), 64, Springer New York LLC

1996

DOI: https://doi.org/10.1007/978-1-4615-5847-7_32

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Abstract

Schiff base

neutron diffraction

collagen fibril

collagen molecule

glycation site

The chemical reactivity of collagen can be studied using neutron diffraction (a non-destructive technique), for certain reaction types. Collagen contains a number of lysine and hydroxylysine side chains that can react with aldehydes and ketones, or these side chains can themselves be converted to aldehydes by lysyl oxidase. The reactivity of these groups not only has an important role in the maintenance of mechanical strength in collagen fibrils, but can also manifest pathologically in the cases of aging, diabetes (reactivity with a variety of sugars) and alcoholism (reactivity with acetaldehyde). The reactivity of reducing groups with collagen can be studied by neutron diffraction, since the crosslink formed in the adduction process is initially of a Schiff base or keto-imine nature. The nature of this crosslink allows it to be deuterated, and the position of this relatively heavy scattering atom can be used in a process of phase determination by multiple isomorphous replacement. This process was used to study the following: the position of natural crosslinks in collagen; the position of adducts in tendon from diabetic rats in vivo and the in vitro position of acetaldehyde adducts in tendon.

Details

Title: The chemical reactivity and structure of collagen studied by neutron diffraction
Authors: Tim J Wess (Author) - University of Stirling, United Kingdom
L Wess (Author) - University of Stirling, United Kingdom
A Miller (Author) - University of Stirling, United Kingdom
Contributors: Benno P Schoenborn (Editor)
Robert B Knott (Editor)
Publication details: Neutrons in Biology, pp.369-383
Series: Basic Life Sciences (BLSC); 64
Publisher: Springer New York LLC
DOI: 10.1007/978-1-4615-5847-7_32
ISBN: 9781461376804
Organisation Unit: University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Academic)
Language: English
Record Identifier: 99450661802621
Output Type: Book chapter

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