Book chapter
Isolation and Detection of Small RNAs from Plant Tissues
Antiviral Resistance in Plants: Methods and Protocols, pp.155-172
Methods in Molecular Biology, 894, Humana Press
2012
PMID: 22678579
Abstract
In plants, several classes of non-coding small RNA (sRNA) have been shown to be important regulators of gene expression in a wide variety of biological processes. The two main classes of sRNA, the small-interfering RNA (siRNA) and microRNA (miRNA) classes, are well documented and several experimental approaches have been developed to allow for their routine isolation and detection from plant tissues. Here, we describe the current methods used for the isolation of total RNA and the subsequent enrichment of low-molecular-weight (LMW) RNA species, as well as to outline how sRNAs are detected from such nucleic acid preparations.
Details
- Title
- Isolation and Detection of Small RNAs from Plant Tissues
- Authors
- Neil A. Smith (Author) - Commonwealth Scientific and Industrial Research OrganisationAndrew Eamens (Author) - University of Sydney
- Contributors
- John M Watson (Editor) - Commonwealth Scientific and Industrial Research OrganisationMing-Bo Wang (Editor) - Commonwealth Scientific and Industrial Research Organisation
- Publication details
- Antiviral Resistance in Plants: Methods and Protocols, pp.155-172
- Series
- Methods in Molecular Biology; 894
- Publisher
- Humana Press
- DOI
- 10.1007/978-1-61779-882-5_11; 10.1007/978-1-61779-882-5
- ISSN
- 1940-6029
- PMID
- 22678579
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health - Biomedicine
- Language
- English
- Record Identifier
- 99656590302621
- Output Type
- Book chapter
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