Channels formed by M2 peptides of a putative glutamate receptor subunit of locust

P N R Usherwood; I Mellor; L Breedon; Robert J Harvey; E A Barnard; M G Darlison

doi:10.1007/978-3-0348-7265-2_12

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Channels formed by M2 peptides of a putative glutamate receptor subunit of locust

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Channels formed by M2 peptides of a putative glutamate receptor subunit of locust

P N R Usherwood, I Mellor, L Breedon, Robert J Harvey, E A Barnard and M G Darlison

Comparative Molecular Neurobiology. EXS, Volume 63, pp.241-249

Birkhaeuser Verlag AG

1993

DOI: https://doi.org/10.1007/978-3-0348-7265-2_12

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Abstract

Pharmacology and Pharmaceutical Sciences

A cDNA encoding part of a polypeptide (Loc1) that exhibits similarity to the corresponding portion of the rat GluRl subunit has been identified by screening an amplified locust cDNA library. This polypeptide is deduced to be missing about 200 amino acids of the amino-terminus and about 100 amino acids of the carboxy-terminus. cDNAs encoding two other glutamate receptor-like polypeptides (Loc2 and Loc3), which both exhibit good sequence homology with Loci, have also been identified. So far, there is no evidence for 'flip' and 'flop' variants of Loci, 2 and 3. A 27-mer peptide including the Ml sequence of Loci and a 25-mer peptide including the M2 sequence of this putative glutamate receptor subunit have been synthesised and incorporated into artificial bilayers. Channel openings, of minimum conductance 20 pS, were seen more frequently with the M2 peptide. These studies are designed to lead to the isolation of full-length cDNAs for Loci, 2 and 3 and to the electrophysiological characterisation of their ion transport properties.

Details

Title: Channels formed by M2 peptides of a putative glutamate receptor subunit of locust
Authors: P N R Usherwood (Author) - University of Nottingham, United Kingdom
I Mellor (Author)
L Breedon (Author)
Robert J Harvey (Author) - MRC Centre, United Kingdom
E A Barnard (Author)
M G Darlison (Author) - MRC Centre, United Kingdom
Contributors: Y Pichon (Editor)
Publication details: Comparative Molecular Neurobiology. EXS, Volume 63, pp.241-249
Publisher: Birkhaeuser Verlag AG
Date published: 1993
DOI: 10.1007/978-3-0348-7265-2_12
ISBN: 9783034872652
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450572302621
Output Type: Book chapter

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