The temporal development of antibody responses to Sarcoptes scabiei infection in a porcine model: relevance to immunodiagnostics

Melanie Rampton; Shelley Walton; Deborah Holt; Cielo Pasay; Bart Currie; James McCarthy; Kate E Mounsey

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The temporal development of antibody responses to Sarcoptes scabiei infection in a porcine model: relevance to immunodiagnostics

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The temporal development of antibody responses to Sarcoptes scabiei infection in a porcine model: relevance to immunodiagnostics

Melanie Rampton, Shelley Walton, Deborah Holt, Cielo Pasay, Bart Currie, James McCarthy and Kate E Mounsey

American Journal of Tropical Medicine and Hygiene, Vol.87(5 supplement)

American Society for Tropical Medicine & Hygiene annual meeting, 2012 (Atlanta, United States, 11-Nov-2012–15-Nov-2012)

2012

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Abstract

No immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei antigen Sar s14.3 is a promising immunodiagnostic, eliciting high levels of IgE in infected patients. Limited data are available regarding the temporal development of antibodies to Sar s14.3, although this is relevant from a clinical perspective. We utilised a porcine model to measure scabies specific antibody levels by ELISA, comparing Sar s 14.3 to S. scabiei whole mite antigen extract (WMA). Robust IgG and IgE responses to both antigens were observed. Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier and declining more rapidly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infection. These data provide important information on the temporal humoral immune responses in scabies and further supports the development of immunodiagnostic tests for scabies.

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Title: The temporal development of antibody responses to Sarcoptes scabiei infection in a porcine model: relevance to immunodiagnostics
Authors: Melanie Rampton (Author) - University of the Sunshine Coast
Shelley Walton (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Deborah Holt (Author) - Menzies School of Health Research
Cielo Pasay (Author) - QIMR Berghofer Medical Research Institute
Bart Currie (Author) - Menzies School of Health Research
James McCarthy (Author) - QIMR Berghofer Medical Research Institute
Kate E Mounsey (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Publication details: American Journal of Tropical Medicine and Hygiene, Vol.87(5 supplement)
Conference details: American Society for Tropical Medicine & Hygiene annual meeting, 2012 (Atlanta, United States, 11-Nov-2012–15-Nov-2012)
Publisher: American Society of Tropical Medicine and Hygiene
Date published: 2012
ISSN: 1476-1645; 0002-9637
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99513869302621
Output Type: Abstract

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