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The effects of sea cucumber extract (Holothuria scabra) on human mesenchymal stem cells derived from placenta
Abstract   Peer reviewed

The effects of sea cucumber extract (Holothuria scabra) on human mesenchymal stem cells derived from placenta

Jutarat Saengsuwan, Napamanee Kornthong, Supawadee Duangprom, Saowaros Suwansa-ard, Sirikul Manochantr and Prasert Sobhon
1st CICM International Conference Abstract and Proceedings Book, p.48
CICM International Conference: Translational Research: From Bench to Bedside, 1st (Pathum Thani, Thailand, 28-Aug-2017–29-Aug-2017)
2017
url
http://eptumed.com/interConference/doc/AbstractandProceeding.pdfView
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Abstract

Fisheries Sciences Holothurian scabra Mesenchymal stromal cells sea cucumber extract cell proliferation
The sea cucumber Holothuria scabra is an economically important aquatic species, which is found in coastal area and naturally distributed in Asian countries, including China, Japan, Malaysia, Thailand, Viet Nam, Indonesia and Philippines. Sea cucumbers have received considerable attention because of their self-regeneration ability. The knowledge of regeneration process, including the factors that regulate this process, may provide a new application to target the treatment of degenerating diseases mammals and humans, especially in cases where the endogenous pathways in mammal may have been lost. This study focused on the effects of sea cucumber extract (H. scabra) on the proliferation of mesenchymal stromal cells (MSCs) derived from human placenta. The H. scabra crude protein extracts were prepared from the body wall (BW) and viscera (VI) by using different extraction buffers, including 0.1M phosphate buffer saline (PBS) and 0.1M acetic acid buffers. The SDS-PAGE showed protein abundance, with various molecular mass, within the BW and VI extracts using 0.1M PBS buffer. Less protein abundance was observed for all organ extracts using 0.1M acetic acid buffer. However, proteins with molecular mass of ~38 kDa and ~17 kDa were highly detected in the extracts from different organs investigated. The MSCs were isolated from the human placentas and were then treated with different doses of sea cucumber extracts. The cytotoxicity and cell proliferation after treatments were evaluated using MTT assay. The results indicated that H. scabra protein extracts at low doeses did not show toxic effect to MSCs, while they could increase the cell number at the range of 0.01 µg/ml to 25 µg/ml. We found that the treatment of 0.1 and 1 µg/ml of H. scabra extracts increased the proliferative rate of MSCs when compared with the sham. This suggested an in vitro proliferative potency of the sea cucumber extracts on MSCs derived from the human placenta. While further studies are required, this finding has firstly provided the evidence that the sea cucumber extracts could be potentially used to induce in vitro MSC proliferation

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