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The Sheep as a Transfusion Model: Comparison of the Storage Lesion of Human and Ovine Red Blood Cell Units
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The Sheep as a Transfusion Model: Comparison of the Storage Lesion of Human and Ovine Red Blood Cell Units

J P Tung, G Simonova, K Glenister, J F Fraser and Yoke Lin Fung
Transfusion medicine, Vol.23(Supplement 2), p.32
Annual Scientific Meeting of the British Blood Transfusion Society, XXXI (Birmingham, United Kingdom, 16-Oct-2013–18-Oct-2013)
2013
DOI: https://doi.org/10.1111/tme.12071
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https://doi.org/10.1111/tme.12071View
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Abstract

Clinical Sciences
Introduction: There is growing awareness of risks related to transfusion of stored blood products. Animal models (e.g. ovine) will bevital to investigating the underlying mechanisms. This study aimed to characterise specific aspects of the storage lesion of ovine (ov) packed red blood cell (PRBC) units and compare these to equivalent human PRBC (huPRBC) units, expanding upon our previous reports of biochemical changes and haemolysis rates. Methods: Whole blood was collected from adult Merino ewes (n=5) into blood packs containing citrate-phosphate-dextrose (CPD) and processed into leucodepleted ovPRBCs preserved in saline, adenine, glucose and mannitol (SAGM) additive solution. Equivalent huPRBCs (n=5) were provided by the Australian Red Cross Blood Service. OvPRBCs and huPRBCs were stored at 2-6 â—¦ C for 42 days, and samples were collected aseptically at weekly intervals (days 1, 7, 14, 21, 28, 35 and 42). Samples underwent testing for 2,3-diphosphoglycerate (2,3- DPG) and adenosine triphosphate (ATP) levels using commercial assays (Roche). Haemoglobin levels were determined using a blood gas analyser (Radiometer ABL System 625). Results: ATP concentrations in both huPRBC and ovPRBC units decreased with storage duration. In huPRBC units, ATP decreased significantly from 8.06±5.74 (mean±standard deviation; μmol / g Hb) at day 1, to (2.30±1.07; P less than 0.05 vs. day 1) at day 35, and continued to decrease to 0.85±0.43 (P less than 0.05 vs. day 35) at day 42. In ovPRBC units, the decrease in ATP from day 1 (9.40±2.72) manifested earlier (day 7 ATP: 5.79±0.51; P less than 0.001), however did not further decrease from this time-point. While the 2,3-DPG concentrations in both huPRBC and ovPRBC units decreased with storage duration, this only reached significance for the huPRBC units. In huPRBC units, 2,3- DPGwas decreased from day 1 (2.99±1.63; mean±standard deviation; μmol / g Hb) at days 14, 35 and 42 (all P less than 0.05), reaching a nadir of 0.70±0.42 at day 42. However, in ovPRBC units, the 2,3-DPG decrease from 1.56±0.62 at day 1, to 0.90±0.39 at day 42 was not significant. Conclusions: Storage induced decreases in ATP and 2,3-DPG concentrations were observed in both huPRBC and ovPRBC units. However, variability in the dynamics of these storage-related changes should be considered when designing transfusion studies in sheep and in extrapolating findings from such studies.

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