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Soluble factors from packed red blood cells augmented LPS Induced monocyte production of IL-1Β and caspase-1
Abstract   Peer reviewed

Soluble factors from packed red blood cells augmented LPS Induced monocyte production of IL-1Β and caspase-1

Robert Flower, Fenny Chong, Kelly Rooks and Melinda M Dean
Pathology, Vol.52(Supplement 1), p.S119
2020
DOI: https://doi.org/10.1016/j.pathol.2020.01.405
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https://doi.org/10.1016/j.pathol.2020.01.405View
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Abstract

Clinical Sciences
Introduction: During routine storage packed red blood cells (PRBC) undergo biochemical and morphological changes, and soluble mediators accumulate which have been hypothesised to contribute to poor outcomes post-transfusion. Interleukin (IL)-1β is a pro-inflammatory cytokine critical for cell proliferation and differentiation. We investigated whether PRBC transfusion modulated IL-1β driven inflammation and assessed whether macrophage inhibitory factor (MIF) was involved. Methods: Isolated monocytes were co-incubated with PRBC supernatants (collected at D2, D14, D28, D42) +/- lipopolysaccharide (LPS, 1 μg/mL) or LPS+ATP (positive control) for 4 hours, 37°C, 5% CO2. IL-1β and caspase-1 were quantified in PRBC-SN and culture SN. MIF was quantified in PRBC-SN and then recombinant MIF (rMIF) +/- LPS added to model 2-3 unit of transfusion. Results were analysed by ANOVA (compared to untreated or LPS alone, p<0.05). Results: PRBC-SN increased LPS-induced monocyte production of IL-1β (p<0.05) and caspase-1 (p<0.05). MIF was present in D2 (19 ng/mL) and D42 (247 ng/mL) PRBC-SN. rMIF did not modulate LPS-induced IL-1β or caspase-1 production from monocytes. Conclusions: Soluble factors in PRBC augmented LPS-induced IL-1β and caspase-1 production in monocytes suggesting inflammasome activation. There was no evidence that MIF was responsible for modulating IL-1β production and further investigation into the other immunomodulatory mediators from PRBC is warranted.

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