Abstract
Reproductive rate and interbreeding of Lineage B and D nematode strains and the implications for Sirex Management
Proceedings of the IUFRO WP 07.02.13 and 07.02.03 joint meeting: Novel and classical strategies to manage forest health in plantations
Joint meeting IUFRO WP 07.02.13 and 07.02.03, 2nd (Camp Grande, Brazil, 17-Sep-2023 - 21-Sep-2023)
2023
Abstract
A recent research study on Australian populations of the parasitic nematode Deladenus siricidicola revealed the dominance of a previously unknown lineage (called "Lineage D") in Sirex noctilio (woodwasp) populations, despite annual introductions of large numbers of the biological control strain of the nematode (“Kamona” or “Lineage B”). This project aimed to compare reproductive rate (number of offspring) between Lineage B and D, and determine their ability to interbreed by pairing in culture. Reproduction rate of D. siricidicola strains from both lineages was compared on four strains of Amylostereum areolatum, on which the nematode feeds in its non-parasitic phase, and different media (MEA and PDA). There was a significant variation in the rate of reproduction between the D. siricidicola strains feeding on four A. areolatum strains. The study found that overall, Lineage D strains exhibited a higher reproductive rate (up to 14 times) compared to Lineage B strains on all A. areolatum strains and media types, offering a plausible explanation for the enduring presence of Lineage D strains in the field. The effect was, however, not significant in all repetitions of the experiment. Interestingly, the commercially used Kamona strain had one of the lowest reproductive rates amongst the strains tested. When Lineage B and D were paired on the same plate, they interbreed and produced heterozygous individuals ranging from 0-98% in the F1 and 37-100% in the F2. The absence of mating barriers suggest that the genetic isolation observed in the field is possibly as a result of ecological, postzygotic or operational factors rather than mating or somatic incompatibility. The results emphasize the importance of considering diversity and nematode-fungus interaction patterns during the mass production of nematodes, and the value of population genetics in managing biological control programs more effectively.
Details
- Title
- Reproductive rate and interbreeding of Lineage B and D nematode strains and the implications for Sirex Management
- Authors
- Katrin N. E. Fitza (Author) - University of PretoriaFirehiwot B. Eshetu (Author) - University of PretoriaHelen Nahrung (Author) - University of the Sunshine Coast, Queensland, Forest Research InstituteIrene Barnes (Author) - University of PretoriaAngus J Carnegie (Author) - New South Wales Department of Primary IndustriesBernard Slippers (Author) - University of Pretoria
- Publication details
- Proceedings of the IUFRO WP 07.02.13 and 07.02.03 joint meeting: Novel and classical strategies to manage forest health in plantations
- Conference details
- Joint meeting IUFRO WP 07.02.13 and 07.02.03, 2nd (Camp Grande, Brazil, 17-Sep-2023 - 21-Sep-2023)
- Publisher
- International Union of Forest Research Organisations
- Organisation Unit
- Forest Industries Research Centre; Forest Research Institute
- Language
- English
- Record Identifier
- 99991497802621
- Output Type
- Abstract
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