Abstract
Prevalence of Strongyloides Stercoralis infection in a remote Indigenous community as determined by ELISA testing from dried blood spot for antibodies to the recombinant antigen NIE
American Journal of Tropical Medicine and Hygiene, Vol.89(5), p.150
American Society of Tropical Medicine and Hygiene (ASTMH) Annual Meeting, 62nd (Washington DC, United States, 13-Nov-2013–17-Nov-2013)
2013
Abstract
Parasitologic diagnosis of infection with the intestinal nematode Strongyloides stercoralis is relatively insensitive and logistically challenging. Serologic assays based on detection of antibodies to crude larval antigen offer increased sensitivity, but specificity is hampered by cross-reactive antibodies and persistence after cure. Further, standardization of antigen for assays is problematic. The use of recombinant antigen can potentially overcome these problems, and the NIE antigen from S.stercoralis has been used widely with good diagnostic sensitivity and specificity. Detection of antibody eluted from dried blood spots has shown utility in large-scale seroepidemiologic studies, and is appealing in children where venipuncture or stool collection is problematic. We adapted an existing NIE-ELISA protocol for the testing of anti-strongyloides antibody response on dried blood spots collected as part of an ivermectin mass drug administration conducted in an Australian Indigenous community. The NIE ELISA was first validated using representative positive, negative, and equivocal timematched serum samples previously tested using S. ratti antigen ELISA. Optimal assay and storage conditions were determined using positive control blood spots, and samples screened with the adapted NIE-DBSELISA. Blood spots were stable for several days at 37oC, or following longer-term storage at 4oC, -20oC or -80oC. The sensitivity of the NIEDBS- ELISA was determined by ROC analysis to be 82%. Of the 219 blood spots tested, 18% were positive for S. stercoralis, a similar prevalence to that documented by standard S. ratti serology. In representative samples positive for S. ratti-specific antibodies, a significant decline in NIE optical density was observed at 6 and 12 months following ivermectin MDA (p less than 0.0001). No time-associated differences were seen in negative or equivocal samples. This further confirms the high seroprevalence of S. stercoralis in remote Australian Indigenous communities, and suggests that collection of dried blood spots may be useful approach for field-friendly diagnosis of strongyloidiasis.
Details
- Title
- Prevalence of Strongyloides Stercoralis infection in a remote Indigenous community as determined by ELISA testing from dried blood spot for antibodies to the recombinant antigen NIE
- Authors
- Kate E Mounsey (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMelanie Rampton (Author) - Queensland Institute of Medical ResearchStacey Llewellyn (Author) - Queensland Institute of Medical ResearchCielo Pasay (Author) - Queensland Institute of Medical ResearchMallory King (Author) - Queensland Institute of Medical ResearchDeborah Holt (Author) - Menzies School of Health ResearchBart Currie (Author) - Menzies School of Health Research
- Publication details
- American Journal of Tropical Medicine and Hygiene, Vol.89(5), p.150
- Conference details
- American Society of Tropical Medicine and Hygiene (ASTMH) Annual Meeting, 62nd (Washington DC, United States, 13-Nov-2013–17-Nov-2013)
- Publisher
- American Society of Tropical Medicine and Hygiene
- Date published
- 2013
- ISSN
- 0002-9637
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449093302621
- Output Type
- Abstract
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