Preliminary Findings from an Ovine Model of Blood Component Therapy Developed to Investigate Transfusion-Related Acute Lung Injury

J P Tung; Yoke Lin Fung; M Nataatmadja; K Colebourne; P McMurray; H Mohamed; K Wilson; P Wood; C C Silliman; J F Fraser

doi:10.1111/j.1365-3148.2010.01002.x

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Preliminary Findings from an Ovine Model of Blood Component Therapy Developed to Investigate Transfusion-Related Acute Lung Injury

Abstract

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Preliminary Findings from an Ovine Model of Blood Component Therapy Developed to Investigate Transfusion-Related Acute Lung Injury

J P Tung, Yoke Lin Fung, M Nataatmadja, K Colebourne, P McMurray, H Mohamed, K Wilson, P Wood, C C Silliman and J F Fraser

Transfusion Medicine, Vol.20(3), pp.203-219

Australian and New Zealand Society of Blood Transfusion (ANZSBT) Annual Scientific Meeting, 2009 (Adelaide, Australia, 18-Oct-2009–21-Oct-2009)

2010

DOI: https://doi.org/10.1111/j.1365-3148.2010.01002.x

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Abstract

Clinical Sciences

Aim To develop an ovine model of blood component therapy (BCT). The model will be used to study adverse reactions including transfusion-related acute lung injury. Methods Sheep were first infused with either saline (healthy sheep) or lipopolysaccharides (LPS) (sepsis sheep). An hour after the first infusion was completed sheep were infused with BCT of either saline (control), day-1 pool of heat-treated supernatants from human wholeblood platelets (d1-S/N) or day-5 pool (d5-S/N). Results LPS-infusion resulted in decreased circulating neutrophils (PMNs) corresponding with pulmonary sequestration of PMNs. Infusion of d1- and d5-S/N into healthy sheep resulted in increased circulating PMN counts. Infusion of human BCT caused only mild pulmonary injury which was most severe when d5-S/N was infused into septic sheep. D5-S/N also caused mild injury (average injury ≥1) more often than d1-S/N. Conclusion These preliminary data indicate that sheep tolerate the infusion of human platelet supernatants, and therefore are potentially a suitable large animal model to study the effects of BCT. Mild pulmonary injury seen more often with stored blood product (d5-SN) may represent a mild TRALI, however further study is required to confirm this.

Details

Title: Preliminary Findings from an Ovine Model of Blood Component Therapy Developed to Investigate Transfusion-Related Acute Lung Injury
Authors: J P Tung (Author) - University of Queensland
Yoke Lin Fung (Author) - University of Queensland
M Nataatmadja (Author) - University of Queensland
K Colebourne (Author) - Australian Red Cross Blood Service
P McMurray (Author) - Australian Red Cross Blood Service
H Mohamed (Author) - Prince Charles Hospital
K Wilson (Author) - Prince Charles Hospital
P Wood (Author) - University of Queensland
C C Silliman (Author) - Bonfils Blood Center, United States
J F Fraser (Author) - University of Queensland
Publication details: Transfusion Medicine, Vol.20(3), pp.203-219
Conference details: Australian and New Zealand Society of Blood Transfusion (ANZSBT) Annual Scientific Meeting, 2009 (Adelaide, Australia, 18-Oct-2009–21-Oct-2009)
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2010
DOI: 10.1111/j.1365-3148.2010.01002.x
ISSN: 0958-7578
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99448857502621
Output Type: Abstract

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Web Of Science research areas: Hematology