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Molecular Methods for Quantification of P. Falciparum and Gametocyte Detection
Abstract   Peer reviewed

Molecular Methods for Quantification of P. Falciparum and Gametocyte Detection

P M Griffin, R J Rockett, K R Trenholme, T P Sloots, S Sekuloski, Kate E Mounsey, S Llewellyn and J S McCarthy
Pathology, Vol.45(Supplement 1), p.S52
Annual Scientific Meeting of the Australasian Division of the International Academy of Pathology Limited (IAP), 37th (Sydney, Australia, 01-Jun-2012–03-Jun-2012)
2013
DOI: https://doi.org/10.1097/01.PAT.0000426847.02436.89
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https://doi.org/10.1097/01.PAT.0000426847.02436.89View
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Abstract

Clinical Sciences blood stage parasites
Despite a recent decline in the burden of malaria worldwide, it continues to cause significant morbidity and mortality. Many new antimalarials are in development, however there are a number of issues that continue to make their assessment problematic. To address this, a human challenge model was developed where healthy subjects are inoculated with blood stage parasites and treated with licensed and candidate antimalarials.1 To be conducted safely a sensitive quantitative malaria hydrolysis probe PCR assay was developed. 2 Another major issue remains the lack of reliable diagnostic methods and limited treatments for the infective stage, the gametocyte. To enable the detection of this stage of the parasite a reverse transcriptase PCR was developed, targeting two stage specific genes, to enable gametocyte kinetics and anti-gametocidal drug activity to be studied.

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