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In search of biomarkers for dietary fat intake: are phospholipids in red blood cell (RBC) membranes a possible candidate?
Abstract

In search of biomarkers for dietary fat intake: are phospholipids in red blood cell (RBC) membranes a possible candidate?

Mark A Holmes, Elizabeth Johnson, Peter R Brooks and Anne H Neller
2007 University Research Conference Book of Abstracts, p.10
University Research Conference: Advancing Sustainable Regions, 2007 (Sunshine Coast, Australia, 21-Nov-2007)
2007
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Abstract

Nutrition and Dietetics Public Health and Health Services dietary fat intake chronic disease
Fat is one of the most difficult dietary components to analyse using traditional dietary intake methods. Because of the suspected link between dietary fat intake and the premature onset of chronic diseases such as cardiovascular disease (CVD), there is now considerable interest in the development of suitable biomarkers for assessing the type and quantity of fat in an individual's diet. This presentation will outline preliminary work undertaken at use to investigate the relationship between the composition of fatty acids in RBC membrane phospholipids, and the dietary fat intake and biomarkers of CVD risk in a sample of apparently healthy adults. Overnight fasted blood samples were collected from 38 adults (28 females, 10 males; 19-55 years of age) on two mornings (T1 and T2) separated by four months. A blood serum lipid profile for each subject was analysed at both blood collections, and their usual food intake during the study period assessed at T2 using a validated food frequency questionnaire. The fatty acid composition of RBC membrane phospholipids was determined using gas chromatographymass spectrometry. The findings from this study, including the observed association between the fatty acid composition of RBC membrane phospholipids in the subjects and their dietary intake of the 'cardioprotective' omega-3 fatty acids, will be described during the presentation. The study was funded through the University Research Grant scheme.

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