Gene Expression Analysis of Hepatopancreas Tissue from Families of Farmed Banana Shrimp (Fenneropenaeus merguiensis) that Exhibit Differing Levels of Hepatopancreatic Parvo-Like Virus Infection

Daniel Powell; Wayne R Knibb; Abigail Elizur

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Gene Expression Analysis of Hepatopancreas Tissue from Families of Farmed Banana Shrimp (Fenneropenaeus merguiensis) that Exhibit Differing Levels of Hepatopancreatic Parvo-Like Virus Infection

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Gene Expression Analysis of Hepatopancreas Tissue from Families of Farmed Banana Shrimp (Fenneropenaeus merguiensis) that Exhibit Differing Levels of Hepatopancreatic Parvo-Like Virus Infection

Daniel Powell, Wayne R Knibb and Abigail Elizur

Proceedings of the 24th Plant and Animal Genome Conference

Plant and Animal Genome (PAG) Conference, 24th (San Diego, United States, 08-Jan-2016–13-Jan-2016)

2016

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Abstract

Fisheries Sciences

Genetics

Viral pathogens pose a serious threat to the cultured shrimp industry. Hepatopancreatic parvo-like virus (HPV) is a shrimp pathogen that has been reported to be widely distributed in a variety of wild and cultured penaeid shrimp species throughout the world. The occurrence of HPV infection was examined in a population of commercially produced banana shrimp (F. merguiensis) in North Queensland, Australia. Large differences (up to 3 orders of magnitude) were observed in HPV copy numbers between families bred and grown together. To further investigate the genetic mechanisms of HPV resistance, hepatopancreas tissue sampled from 4 animals from each of 6 families (3 exhibiting high viral load and 3 exhibiting lower viral load, n=24), were subjected to RNA-Seq, de-novo transcriptome assembly and subsequent gene expression analysis. These data revealed over 400 transcripts that were differentially expressed between the high and low families and uncovered a rich set of genes involved in immune system-related functions. Over 80 of these differentially expressed transcripts exhibited homology with genes associated with invertebrate innate immune responses to known bacterial and viral pathogens. Comparative analysis of gene sequences among family groups revealed a number of interesting single nucleotide variations (SNVs). This research provided some insight into our understanding of the mechanisms involved in the response of this shrimp species to a naturally occurring viral pathogen. SNVs identified in this study may be further investigated to determine their efficacy as a mechanism of selection for families with a higher resistance to infection from this pathogen.

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Title: Gene Expression Analysis of Hepatopancreas Tissue from Families of Farmed Banana Shrimp (Fenneropenaeus merguiensis) that Exhibit Differing Levels of Hepatopancreatic Parvo-Like Virus Infection
Authors: Daniel Powell (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Wayne R Knibb (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Proceedings of the 24th Plant and Animal Genome Conference
Conference details: Plant and Animal Genome (PAG) Conference, 24th (San Diego, United States, 08-Jan-2016–13-Jan-2016)
Publisher: Plant and Animal Genome (PAG) Conference
Date published: 2016
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Office of Research; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450092902621
Output Type: Abstract

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