Abstract
Fishing out uniquely expressed genes of the androgenic gland in the Australian crayfish Cherax quadricarinatus
Annual Meeting of the Society for Integrative and Comparative Biology (SICB), 2006 (Orlando, United States, 04-Jan-2006 - 08-Jan-2006)
2006
Abstract
The Australian crayfish Cherax quadricarinatus is a gonochoristic decapod species in which the androgenic gland (AG), an organ unique to male crustaceans, regulates sex differentiation, sexual secondary characteristics, and behavior. It is thought that the AG exerts its effects through androgenic gland hormone(s), not yet identified in decapods, whose synthesis and secretion are mediated by AG uniquely expressed genes. In order to identify such genes, a subtractive cDNA library of C. quadricarinatus AGs was constructed using cDNA from AGs as the "tester" and cDNA from other peripheral glands as the subtractive reference. cDNA products of the subtractive library were cloned, sequenced and an automated BLAST-X search suggested similarity to other known proteins in different organisms. So far, 200 sequences were analyzed and assembled into 49 putative genes, part of them could be assigned to metabolic pathways and a few were putatively identified as structural proteins. The majority of the putative genes showed no significant similarity to any known proteins in the Swiss-Prot database. Twenty of the above transcripts were tested by RT-PCR and two confirmed to be uniquely expressed in the AG. Northern blot analysis of these two genes revealed transcripts of 1000 and 2000 bp. Seeking full sequence of these uniquely expressed genes will help in identification of their functions.
Details
- Title
- Fishing out uniquely expressed genes of the androgenic gland in the Australian crayfish Cherax quadricarinatus
- Authors
- R Manor (Author) - Ben-Gurion University of the Negev, IsraelS Weil (Author) - Ben-Gurion University of the Negev, IsraelS Oren (Author) - Ben-Gurion University of the Negev, IsraelE D Aflalo (Author) - Ben-Gurion University of the Negev, IsraelTomer Ventura (Author) - Ben-Gurion University of the Negev, IsraelM Lapidot (Author) - Ben-Gurion University of the Negev, IsraelV Chalifa-Caspi (Author) - Ben-Gurion University of the Negev, IsraelA Sagi (Author) - Ben-Gurion University of the Negev, Israel
- Conference details
- Annual Meeting of the Society for Integrative and Comparative Biology (SICB), 2006 (Orlando, United States, 04-Jan-2006 - 08-Jan-2006)
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99450287602621
- Output Type
- Abstract
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