Culture independent detection of chlorhexidine resistance genes qacA/B and smr in bacterial DNA recovered from body sites treated with chlorhexidine containing dressings

Md Abu Choudhury; Hanna Sidjabat; Irani Rathnayake; Nicole Gavin; Raymond Chan; Shahera Banu; Flavia Huygens; David Paterson; Claire Rickard; David J McMillan

doi:10.1016/j.idh.2016.09.030

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Culture independent detection of chlorhexidine resistance genes qacA/B and smr in bacterial DNA recovered from body sites treated with chlorhexidine containing dressings

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Culture independent detection of chlorhexidine resistance genes qacA/B and smr in bacterial DNA recovered from body sites treated with chlorhexidine containing dressings

Md Abu Choudhury, Hanna Sidjabat, Irani Rathnayake, Nicole Gavin, Raymond Chan, Shahera Banu, Flavia Huygens, David Paterson, Claire Rickard and David J McMillan

Infection, Disease & Health, Vol.21(3), p.122

International Australasian College for Infection Prevention and Control (ACIPC) Conference, 5th (Melbourne, Australia, 20-Nov-2016–23-Nov-2016)

2016

DOI: https://doi.org/10.1016/j.idh.2016.09.030

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Abstract

Medical Microbiology

Immunology

Chlorhexidine gluconate (CHG) containing dressings are increasingly used in clinical environments for prevention of infection at central venous catheter insertion sites. Increased tolerance to this biocide in Staphylococci is primarily associated with the presence of qacA/B and smr genes. Here we used a culture independent method to assess the prevalence of these genes in 78 DNA specimens recovered from the skin of 43 patients at catheter insertion sites in the arm that were covered with CHG-dressings. Of the 78 DNA specimens analysed, 52 (67%) possessed qacA/B and 14(18%) contained smr; all sample positive for smr were also positive for qacA/B. These prevalence rates was not statistically greater than that observed in a subsample of specimens taken from non-CHG treated arms. A statistically greater proportion of specimens with greater than 72 hours exposure to CHG dressings were qac-positive (pZ 0.04), suggesting the patients were contaminated with bacteria or DNA containing qacA/B during their hospital stay. The presence of qacA/B was also positively associated with the presence of DNA specific for S epidermidis and S. aureus in these specimens. Our results show that CHG-genes are highly prevalent on hospital patients' skin, even in the absence of viable bacteria.

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Title: Culture independent detection of chlorhexidine resistance genes qacA/B and smr in bacterial DNA recovered from body sites treated with chlorhexidine containing dressings
Authors: Md Abu Choudhury (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Hanna Sidjabat (Author) - University of Queensland
Irani Rathnayake (Author) - Queensland University of Technology
Nicole Gavin (Author) - Griffith University
Raymond Chan (Author) - Griffith University
Shahera Banu (Author) - Queensland University of Technology
Flavia Huygens (Author) - Queensland University of Technology
David Paterson (Author) - University of Queensland
Claire Rickard (Author) - Griffith University
David J McMillan (Author) - Griffith University
Publication details: Infection, Disease & Health, Vol.21(3), p.122
Conference details: International Australasian College for Infection Prevention and Control (ACIPC) Conference, 5th (Melbourne, Australia, 20-Nov-2016–23-Nov-2016)
Publisher: Elsevier BV
Date published: 2016
DOI: 10.1016/j.idh.2016.09.030
ISSN: 2468-0451
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451068602621
Output Type: Abstract

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