Characterisation of CD4+ T cells in healthy and diseased koalas (Phascolarctos cinereus) using cell-type-specific monoclonal antibodies

C Mangar; C W Armitage; Peter Timms; L M Corcoran; K W Beagley

doi:10.1002/eji.201670200

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Characterisation of CD4+ T cells in healthy and diseased koalas (Phascolarctos cinereus) using cell-type-specific monoclonal antibodies

Abstract

Peer reviewed

Characterisation of CD4+ T cells in healthy and diseased koalas (Phascolarctos cinereus) using cell-type-specific monoclonal antibodies

C Mangar, C W Armitage, Peter Timms, L M Corcoran and K W Beagley

European Journal of Immunology, Vol.46(Supplement 1), p.465

International Congress of Immunology (ICI), 2016 (Melbourne, Australia, 21-Aug-2016–26-Aug-2016)

2016

DOI: https://doi.org/10.1002/eji.201670200

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Abstract

Immunology

The koala (Phascolarctos cinereus) is an arboreal herbivorous marsupial that is an Australian icon. Chlamydia pecorum and C. pneumoniae are obligate intracellular Gram-negative bacteria that infect koalas and can cause, infertility, arthritis, conjunctivitis, respiratory disease, and in some cases death. Due to a lack of koala-specific immune reagents and assays there is currently no way to adequately analyse the immune response in diseased, healthy or vaccinated animals. Using the extracellular domain of koala CD4+ this paper reports the production and characterisation of the first anti-koala CD4 monoclonal antibody (mAb). Fluorochrome-conjugated anti-CD4 mAb was used to investigate the levels of CD4+ T helper cells collected from koala spleens, lymph nodes and peripheral blood by flow cytometry. Biotin-conjugated anti-CD4 mAb was also used for Western Blot and immunohistochemistry to identify CD4+ T helper cells in the lymph node. The results indicate that CD4+ specific T cells could be identified using flow cytometry of cells from both tissues and peripheral blood as well as visualised using immunohistochemistry techniques. Further analysis of the results also showed an increased level of CD4+ cells in animals with current signs of chlamydial infection/disease as opposed to those showing no signs of infection or clinical disease. Since CD4+ T cells have been shown to play a pivotal role in clearing chlamydial infection in both human and mouse infections, using this novel antibody will help determine the role CD4+ T cells play in protection against chlamydial infection in koalas.

Details

Title: Characterisation of CD4+ T cells in healthy and diseased koalas (Phascolarctos cinereus) using cell-type-specific monoclonal antibodies
Authors: C Mangar (Author) - Queensland University of Technology
C W Armitage (Author) - Queensland University of Technology
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
L M Corcoran (Author) - Walter and Eliza Hall Institute of Medical Research (WEHI)
K W Beagley (Author) - Queensland University of Technology
Publication details: European Journal of Immunology, Vol.46(Supplement 1), p.465
Conference details: International Congress of Immunology (ICI), 2016 (Melbourne, Australia, 21-Aug-2016–26-Aug-2016)
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
Date published: 2016
DOI: 10.1002/eji.201670200
ISSN: 0014-2980
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99451294502621
Output Type: Abstract

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