Application of Diversity Arrays Technology (DArT) in Eucalyptus phylogeny reconstruction: from populations to species

Dorothy A Steane; A Myburg; A Kilian; J Carling; E Huttner; C P Sansaloni; C D Petroli; D Grattapaglia; R E Vaillancourt

Application of Diversity Arrays Technology (DArT) in Eucalyptus phylogeny reconstruction: from populations to species

Abstract

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Application of Diversity Arrays Technology (DArT) in Eucalyptus phylogeny reconstruction: from populations to species

Dorothy A Steane, A Myburg, A Kilian, J Carling, E Huttner, C P Sansaloni, C D Petroli, D Grattapaglia and R E Vaillancourt

BioBuzz, (11)

IUFRO Kuala Lumpur Conference, 2010 (Kuala Lumpur, Malaysia, 07-Mar-2010–12-Mar-2010)

2010

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Abstract

Forestry Sciences

Diversity Arrays Technology (DArT)

eucalyptus

phylogeny

population genetics

Eucalyptus L'Herit. is a large economically and ecologically important genus of over 700 species, endemic to Australia and several tropical islands to its north. Eucalypts are planted worldwide in both tropical and temperate regions for timber and pulp production. Long term sustainable management of the resource requires a detailed understanding of the genetic diversity within and between species in native populations. Eucalyptus taxonomy is confounded by high levels of quantitative variation and homoplasy among morphological characters, low levels of phylogenetically useful variation in ribosomal and chloroplast DNA, and incomplete reproductive isolation of species that results in interspecific hybridisation and introgression. Diversity Arrays Technology (DArT) markers are highly variable genomewide binary markers that are based on restriction site polymorphisms detected by DNA hybridization on microarrays. Originally developed for use in population genetics, association genetics and genome mapping, DArTs are proving to be highly informative about phylogenetic relationships at all taxonomic levels within Eucalyptus. We have developed an array of highly polymorphic, highly transferable DArT markers from a range of eucalypt species. Phylogenetic analyses using over 8000 DArT markers resolved relationships among subgenera, sections and species of Eucalyptus. DArT markers also detected genetic subdivision in species that have geographically disjunct populations (e.g., E. urophylla, E. camaldulensis, E. globulus and E. cladocalyx). The general congruence of the results with those obtained from traditional morphology-based classifications, or from ITS, AFLP or SSR data gives us confidence that genome-wide genotyping with DArT will allow rapid and relatively inexpensive resolution of previously intractable phylogenetic questions within this globally important genus.

Details

Title: Application of Diversity Arrays Technology (DArT) in Eucalyptus phylogeny reconstruction: from populations to species
Authors: Dorothy A Steane (Author) - University of Tasmania
A Myburg (Author) - University of Pretoria, South Africa
A Kilian (Author) - Diversity Arrays Technology Pty Ltd, Australia
J Carling (Author) - Diversity Arrays Technology Pty Ltd, Australia
E Huttner (Author) - Diversity Arrays Technology Pty Ltd, Australia
C P Sansaloni (Author) - Universidade de Brasilia, Brazil
C D Petroli (Author) - Universidade de Brasilia, Brazil
D Grattapaglia (Author) - Universidade Catolica de Brasília, Brazil
R E Vaillancourt (Author) - University of Tasmania
Publication details: BioBuzz, (11)
Conference details: IUFRO Kuala Lumpur Conference, 2010 (Kuala Lumpur, Malaysia, 07-Mar-2010–12-Mar-2010)
Publisher: CRC for Forestry
Date published: 2010
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450040002621
Output Type: Abstract

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