A turning point in elastin structure

L B Dyksterhuis; Clair Baldock; Donna Lammie; Tim J Wess; A S Weiss

doi:10.1016/j.matbio.2006.08.049

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Abstract

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A turning point in elastin structure

L B Dyksterhuis, Clair Baldock, Donna Lammie, Tim J Wess and A S Weiss

Matrix Biology, Vol.25(Supplement 1), p.S17

American Society for Matrix Biology (ASMB) Meeting Abstracts, 2006 (Nashville, United States, 01-Nov-2006–04-Nov-2006)

2006

DOI: https://doi.org/10.1016/j.matbio.2006.08.049

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Abstract

Biological Sciences

The central region of human tropoelastin forms a hot_spot for contacts during inter_molecular association by coacervation. We explored the properties of this central region using a sub_fragment bordered by domains 17_27 (SHEL 17_27). A homobifunctional amine reactive cross_linker (BS3) was used to identify the inter_and intra_molecular contacts formed during its coacervation. Cross_linked regions were identified by protease cleavage and mass spectrometry (MS) and verified by MS/MS. Domains 19 and 21_23 were identified as key points of contact during the process of coacervation. Domain 19 formed an intermolecular cross_link to another K353 in all samples investigated, even at low BS3 concentrations, suggesting that this link reports an important initial intermolecular contact for coacervation. K425 and K437 of domain 21_23 showed a high level of involvement in intermolecular contacts at higher BS3 concentrations, suggesting solvent exposure and/or flexibility in this domain. Additionally, an intramolecular cross_link between K425 and K437 formed over a distance that exceeded the maximum span of BS3 (7 residues), providing the first direct evidence for a bend at this unusual crosslinking location. Small_angle x_ray scattering data provided dimensional information for the domain and confirmed the presence of a bend. While numerous turns are thought to exist in the hydrophobic domains of tropoelastin, this is the only turn to be identified between the lysines of a crosslinking domain. The importance of this turn to the function of tropoelastin is discussed.

Details

Title: A turning point in elastin structure
Authors: L B Dyksterhuis (Author) - University of Sydney
Clair Baldock (Author) - University of Manchester, United Kingdom
Donna Lammie (Author) - Cardiff University, United Kingdom
Tim J Wess (Author) - Cardiff University, United Kingdom
A S Weiss (Author) - University of Sydney
Publication details: Matrix Biology, Vol.25(Supplement 1), p.S17
Conference details: American Society for Matrix Biology (ASMB) Meeting Abstracts, 2006 (Nashville, United States, 01-Nov-2006–04-Nov-2006)
Publisher: Elsevier BV
Date published: 2006
DOI: 10.1016/j.matbio.2006.08.049
ISSN: 0945-053X
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451244402621
Output Type: Abstract

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Cell Biology