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Multiplex PCRs for Identification of Escherichia coli Virulence Genes
Journal article   Peer reviewed

Multiplex PCRs for Identification of Escherichia coli Virulence Genes

M. A Pass, R Odedra and R. M Batt
Journal of Clinical Microbiology, Vol.38(5), pp.2001-2004
Note
2000
PMCID: PMC86652
PMID: 10790141
url
https://doi.org/10.1128/JCM.38.5.2001-2004.2000View
Published Version Open

Abstract

Bacteriology
PCRs were developed to detect 11 Escherichia colivirulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

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