Files and links (2)
Published VersionCC BY V4.0, Open Access
Journal article
KV7 Channel Expression and Function Within Rat Mesenteric Endothelial Cells
Frontiers in Physiology, Vol.11, pp.1-16
2020
Abstract
Background and Purpose: Arterial diameter is dictated by the contractile state of the vascular smooth muscle cells (VSMCs), which is modulated by direct and indirect inputs from endothelial cells (ECs). Modulators of KCNQ-encoded kV7 channels have considerable impact on arterial diameter and these channels are known to be expressed in VSMCs but not yet defined in ECs. However, expression of kV7 channels in ECs would add an extra level of vascular control. This study aims to characterize the expression and function of KV7 channels within rat mesenteric artery ECs.
Experimental Approach: In rat mesenteric artery, KCNQ transcript and KV7 channel protein expression were determined via RT-qPCR, immunocytochemistry, immunohistochemistry and immunoelectron microscopy. Wire myography was used to determine vascular reactivity.
Key Results: KCNQ transcript was identified in isolated ECs and VSMCs. KV7.1, KV7.4 and KV7.5 protein expression was determined in both isolated EC and VSMC and in whole vessels. Removal of ECs attenuated vasorelaxation to two structurally different KV7.2-5 activators S-1 and ML213. KIR2 blockers ML133, and BaCl2 also attenuated S-1 or ML213-mediated vasorelaxation in an endothelium-dependent process. KV7 inhibition attenuated receptor-dependent nitric oxide (NO)-mediated vasorelaxation to carbachol, but had no impact on relaxation to the NO donor, SNP.
Conclusion and Implications: In rat mesenteric artery ECs, KV7.4 and KV7.5 channels are expressed, functionally interact with endothelial KIR2.x channels and contribute to endogenous eNOS-mediated relaxation. This study identifies KV7 channels as novel functional channels within rat mesenteric ECs and suggests that these channels are involved in NO release from the endothelium of these vessels.
Details
- Title
- KV7 Channel Expression and Function Within Rat Mesenteric Endothelial Cells
- Authors
- Samuel N Baldwin (Author) - St George's, University of LondonShaun L Sandow (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyGema Mondéjar-Parreño (Author) - Universidad Complutense de MadridJennifer B Stott (Author) - St George's, University of LondonIain A Greenwood (Author) - St George's, University of London
- Publication details
- Frontiers in Physiology, Vol.11, pp.1-16
- Publisher
- Frontiers Research Foundation
- Date published
- 2020
- DOI
- 10.3389/fphys.2020.598779
- ISSN
- 1664-042X
- Copyright note
- Copyright © 2020 Baldwin, Sandow, Mondéjar-Parreño, Stott and Greenwood. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99498108002621
- Output Type
- Journal article
Metrics
5 File views/ downloads
43 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Physiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites